miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5

International Journal of Biological Sciences
Jingbang WuShusen Zheng

Abstract

MicroRNAs (miRNAs) have been validated to play prominent roles in the occurrence and development of many kinds of malignant cancer. MiR-424-5p has been reported to participate in various tumors proliferation and metastasis as a suppressor. On the contrary, miR-424-5p would promote cell proliferation in some tumors. However, the expression of miR-424-5p in intrahepatic cholangiocarcinoma (ICC) is rarely reported and its mechanism remains unclear. Here, we discover that miR-424-5p is frequently downregulated in ICC tissues compared with adjacent normal tissues and in ICC cells. Over-expression of miR-424-5p significantly inhibits the invasion and migration of ICC cells in vitro. Importantly, miR-424-5p is found to be a suppressor of ARK5, by binding to 3'-UTR of ARK5 mRNA and then inhibiting mTOR phosphorylated, thus deregulating epithelial-mesenchymal transition (EMT) of ICC. Furthermore, ARK5 is found to play a role in ICC metastasis and regulating EMT. Knockdown of ARK5 inhibits invasion and migration of ICC, while the over-expression gives an opposite effect. Besides, high-expression of ARK5 is also associated with poor prognosis. In conclusion, our study reveals that miR-424-5p is critical to the invasion, migration and EMT ...Continue Reading

Citations

Feb 23, 2020·Expert Opinion on Therapeutic Targets·Keisaku SatoGianfranco Alpini
Apr 3, 2020·International Journal of Oncology·Yu-Xue GaoDe-Xi Chen
Dec 17, 2020·Cell Biology International·Narges DastmalchiReza Safaralizadeh
Oct 17, 2020·OncoTargets and Therapy·Shulin LiXiangwei Wang
Apr 29, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Deng NingLi Jiang
Jul 25, 2021·International Journal of Molecular Sciences·Tingting ShiTsutomu Masaki

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BETA
transfection
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transfecting

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TargetScan

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