MiR-4429 prevented gastric cancer progression through targeting METTL3 to inhibit m6 A-caused stabilization of SEC62

Biochemical and Biophysical Research Communications
Huabo HeLixin Chai

Abstract

Gastric cancer (GC) has been recognized as the major reason for global cancer-associated mortality. SEC62 homolog, preprotein translocation factor (SEC62) has been documented to possess carcinogenic functions in cancers, but its influence on GC remains elusive. Present study aimed to uncover the impact and mechanism of SEC62 in GC. We validated the upregulation of SEC62 in GC samples by GEPIA, and revealed its high level in GC cell lines. Functionally, depletion of SEC62 hindered proliferation and encouraged apoptosis in GC cells. Furthermore, we found through Starbase 3.0 and validated that methyltransferase like 3 (METTL3) interacted with SEC62 to induce the m6A on SEC62 mRNA, therefore facilitated the stabilizing effect of IGF2 binding protein 1 (IGF2BP1) on SEC62 mRNA. Moreover, we predicted through miRmap and validated that miR-4429 targeted and inhibited METTL3 to repress SEC62. Rescue assays demonstrated that miR-4429 inhibited GC progression through METTL3/SEC62 axis. Together, our study firstly revealed that miR-4429 prevented gastric cancer progression through targeting METTL3 to inhibit m6A-caused stabilization of SEC62, indicating miR-4429 as a promising target for treatment improvement for GC.

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Citations

May 15, 2020·Experimental & Molecular Medicine·Seung Hun Han, Junho Choe
May 21, 2020·Journal of Hematology & Oncology·Qiang WangShouyu Wang
Jan 11, 2020·Frontiers in Oncology·Wenhui ZhengMing Gu
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Oct 9, 2020·Cell Proliferation·Fu-Chun HuoDong-Sheng Pei
Feb 27, 2021·Cancer Control : Journal of the Moffitt Cancer Center·KuangZheng LiuMing Chen
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May 1, 2021·Journal of Experimental & Clinical Cancer Research : CR·Fenghua TanCan Guo
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Jun 11, 2021·Cell Death & Disease·Xiao HanZhipeng Fan
Jun 13, 2021·Genomics·Raja Ishaq Nabi Khan, Waseem Akram Malla
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Jul 12, 2021·Experimental Hematology & Oncology·Junchao HuangWendong Gu
Jul 29, 2021·Journal of Hematology & Oncology·Wei HuangYue-Qin Chen
Aug 28, 2021·In Vitro Cellular & Developmental Biology. Animal·Wei LiLiang Wang
Dec 6, 2021·Cell Biology International·Lulu WangXuejun Gao

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