MiR-4463 inhibits the migration of human aortic smooth muscle cells by AMOT

Bioscience Reports
Xueqin WangXiangyu Zhou

Abstract

Aberrant vascular smooth muscle cell (VSMC) migration has been implicated in a variety of vascular disorders, while the signal pathways governing this process remain unclear. Here, we investigated whether miRNAs, which are strong post-transcriptional regulators of gene expression, could alter VSMC migration. We detected the expression of miR-4463 in the plasma of patients with atherosclerosis and in human aortic smooth muscle cells under hypoxia-ischemia condition, and investigated the migration effect and its downstream pathways. The results have shown that whether in clinical AS patients or hypoxic cells, the expression of miR-4463 was lower than that of normal group, then the number of migrating cells in the miR-4463 mimic intervention group was significantly decreased compared with the normal group and miR-4463 inhibitor instead. Furthermore, the expression of angiomotin (AMOT) in gastrocnemius muscle and femoral artery of patients was significantly higher than that of the control group. The protein level of AMOT in miR-4463 mimic intervention group was significantly decreased, and its level was reversed by inhibiting miR-4463. In summary, these results indicate that miR-4463 is a novel modulator of VSMC migration by target...Continue Reading

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Citations

Feb 8, 2020·Andrologia·Zohreh HeidaryHamid Reza Khorram Khorshid
Aug 12, 2020·Journal of Cell Communication and Signaling·Jingbo ChenChaohong Li
Aug 29, 2020·Clinical and Experimental Reproductive Medicine·Su-Yeon LeeMi Kyoung Koong
Nov 28, 2020·Cell and Tissue Research·Xueqin WangXiangyu Zhou

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Methods Mentioned

BETA
flow cytometry
transfecting
PCR
Fluorescence
transfection
amputation

Software Mentioned

TargetScan
StepOnePlus
SPSS
AMOT
cellSens standard
Quantity One

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