miR-448 targets IDO1 and regulates CD8+ T cell response in human colon cancer.

Journal for Immunotherapy of Cancer
Qiong LouHuanliang Liu

Abstract

Indoleamine 2,3-dioxygenase 1 (IDO1) is a critical regulator of T cell function, contributing to immune tolerance. Upregulation of IDO1 has been found in many cancer types; however, the regulatory mechanisms and clinical significance of IDO1 in colon cancer are still unclear. Here, we investigated the role of dysregulated microRNA (miRNA) targeting IDO1 in the colon cancer microenvironment. We elucidated IDO1 function by performing cell-based assays and establishing transplanted tumor models in BALB/c mice and BALB/c nude mice. We evaluated IDO1 protein expression by immunohistochemistry (IHC) in a tissue microarray (TMA) and analyzed IDO1 mRNA expression with The Cancer Genome Atlas (TCGA). We screened miRNAs targeting IDO1 by using a dual luciferase reporter assay. We tested the function of microRNA-448 (miR-448) by using western blotting (WB) and fluorescence-activated cell sorting (FACS). We demonstrated that stable IDO1 overexpression enhanced xenograft tumor growth in BALB/c mice but not in BALB/c nude mice. We also revealed the involvement of posttranscriptional regulation of IDO1 in colon cancer by observing IDO1 protein levels and mRNA levels. Furthermore, ectopic expression of miRNA mimics suggested that miR-448 could...Continue Reading

References

Oct 3, 2002·Nature Reviews. Immunology·Joseph A Trapani, Mark J Smyth
Oct 31, 2002·Nature Immunology·Gavin P DunnRobert D Schreiber
May 10, 2003·Trends in Immunology·Ursula GrohmannPaolo Puccetti
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Ingo KauschAndreas Böhle
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nu-Man TsaiHorng-Jyh Harn
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aikou OkamotoMitsuyoshi Urashima
Feb 21, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gerald BrandacherAlbert Amberger
Oct 3, 2006·Developmental Cell·Wigard P Kloosterman, Ronald H A Plasterk
May 4, 2007·The Journal of Clinical Investigation·David H Munn, Andrew L Mellor
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kazuhiko InoFumitaka Kikkawa
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Norio YoshidaFumitaka Kikkawa
Jul 29, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Babak BabanAndrew L Mellor
Aug 22, 2009·Journal of Translational Medicine·Yan-Fang GaoXiao-Shi Zhang
Oct 29, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Reinhart SpeeckaertLieve Brochez
Aug 31, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Derek A WainwrightMaciej S Lesniak
Jan 4, 2013·Current Opinion in Gastroenterology·Matthew A Ciorba
Aug 30, 2013·Science Translational Medicine·Stefani SprangerThomas F Gajewski
Jan 18, 2014·Molecular Biology Reports·M Cavia-SaizC García Girón
Feb 4, 2016·Trends in Immunology·David H Munn, Andrew L Mellor
Jan 20, 2017·Nature·Daniel S Chen, Ira Mellman
May 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Samaneh KhorramiReza Malekzadeh
Mar 10, 2018·Journal of Cancer Research and Clinical Oncology·Soo Jung LeeJong Gwang Kim
Mar 23, 2018·Journal of Gastrointestinal Oncology·Aparna KalyanAl Benson
Apr 25, 2018·Journal of Hematology & Oncology·Qian HuangYong Li
Jun 11, 2018·International Journal of Biological Macromolecules·Xinhong PeiHuixiang Li
Jul 5, 2018·Journal for Immunotherapy of Cancer·Muhammad Umair MushtaqFotis Asimakopoulos
Jan 6, 2019·Nature Reviews. Drug Discovery·Jérôme Galon, Daniela Bruni

❮ Previous
Next ❯

Citations

Jan 3, 2020·International Journal of Oncology·Zhaoji PanChengsong Cao
Jun 11, 2020·Cancer Cell International·Huixin SongQuan Liao
Dec 25, 2019·Analytical Cellular Pathology (Amsterdam)·Da QinLi Min
Jul 30, 2020·Cancers·Sunglim ChoLi-Fan Lu
Apr 19, 2021·Seminars in Cell & Developmental Biology·Priya MondalSyed Musthapa Meeran
May 1, 2021·Seminars in Cell & Developmental Biology·Saife N LoneMuzafar A Macha
Aug 9, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Behnia AkbariHamid Reza Mirzaei
Aug 28, 2021·International Journal of Molecular Sciences·Daria KoninaMikhail Skoblov
Oct 17, 2021·Journal of Hematology & Oncology·Yiyin ZhangHui Lin

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
transfection
Assay
ELISA
Flow cytometry
xenograft

Software Mentioned

ImageJ
FlowJo
SPSS
Image
xCELLigence analysis ( RTCA )
Pro Plus
Pro
TargetScan
Plus
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Immunotherapy

Cancer immunotherapy is an important field of research that is looking at controlling cancer and tumor growth by activating the individuals own immune system. Recent studies have utilized chimeric antigen receptor t-cell therapy, immune checkpoint inhibitors and neoantigen vaccines. Discover the latest research on cancer immunotherapy here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis