miR-451 selectively increases sensitivity to cisplatin in ERCC1-high non-small cell lung cancer cells

Journal of Cellular Biochemistry
Kaihua LiuKai Ma

Abstract

Lung cancer is the leading cause of cancer-related death throughout the world and cisplatin chemoresistance is one of the major hindrances of efficiency in this malignancy therapy. It has been reported that miR-451, a tumor suppressor, is involved in sensitivity of cancer cells to cisplatin. However, the role of miR-451 in chemosensitivity of lung cancer cells and the underlying mechanism still remains to be not fully illuminated. We first assessed the expression of ERCC1 and miR-451 in six NSCLS cell lines and NHBE cells by qRT-PCR. Then ERCC1-low and ERCC1-high NSCLC cells were transfected with miR-451 mimic and mimic control. And cell viability, apoptotic cell rates, and migration activity was respectively measured by CCK-8 assay, flow cytometry, and wound healing assay. The expression of ERCC1, Wnt/β-catenin and PI3K/AKT pathway related factors were measured by western blot. The expression of miR-451 was higher in ERCC1-low NSCLC cells, while lower in ERCC1-high NSCLC cells. miR-451 overexpression inhibited the expression of ERCC1 in ERCC1-high NSCLC cells. Furthermore, miR-451 overexpression selectively enhanced cisplatin sensitivity in ERCC1-high NSCLC cells, as evidenced by reduction of cell viability with a dose manner....Continue Reading

Citations

Mar 12, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sangwoon ChungJohn W Christman
Jun 26, 2020·Frontiers in Genetics·Liping SunDeqing Wang

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