MiR-455-3p inhibits the degenerate process of chondrogenic differentiation through modification of DNA methylation

Cell Death & Disease
Hao SunZhiqi Zhang

Abstract

The aim of this work was to determine whether miR-455-3p regulates DNA methylation during chondrogenic differentiation of hMSCs. The expression of miR-455-3p and de novo methyltransferase DNMT3A was assessed in micromass culture of hBMSCs, which induced chondrogenic differentiation in vitro, and in E16.5 mice in vivo. A luciferase reporter assay was used to confirm whether miR-455-3p directly targets DNMT3A by interaction with the 3'-UTR. Using an Illumina Infinium Methylation EPIC microarray, genome-wide DNA methylation of hBMSCs with or without overexpressed miR-455-3p was examined for 28 days during induced chondrogenic differentiation. Here, we showed that miR-455-3p was more expressed during the middle stage of hBMSC chondrogenic differentiation, and less expressed in the late stage. DNMT3A was less expressed in the middle stage and more expressed in the late stage, and was also more expressed in the palms of miR-455-3p deletion mice compared to those of wild-type mice. The luciferase reporter assay demonstrated that miR-455-3p directly targets DNMT3A 3'-UTR. miR-455-3p overexpression inhibits the degenerate process during chondrogenic differentiation, while deletion of miR-455-3p in mice accelerated cartilage degeneration...Continue Reading

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Citations

Apr 11, 2020·Nature Reviews. Rheumatology·Sarah J RiceJohn Loughlin
Dec 4, 2019·BMC Musculoskeletal Disorders·Yi LongAishan He
Sep 12, 2019·Frontiers in Cell and Developmental Biology·Guping MaoZhiqi Zhang
Sep 14, 2019·International Journal of Molecular Sciences·Ehsan RazmaraSadegh Babashah
Jan 6, 2021·Journal of Cellular Biochemistry·Yan-Hua DongMing Cang
Feb 23, 2021·Environmental and Molecular Mutagenesis·Katherine F WallisIsabelle R Miousse
Mar 27, 2021·Molecular Genetics and Genomics : MGG·Boyin JiaHengxing Ba
Feb 24, 2021·BioFactors·Sanam DolatiSarvin Sanaie
Jul 30, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kishor PantSergio A Gradilone

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Methods Mentioned

BETA
acetylation
total hip arthroplasty
in vitro transcription
PCR
transfection
electrophoresis

Software Mentioned

GenomeStudio
SPSS
TargetScan

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