miR-486-5p Inhibits Inflammatory Response, Matrix Degradation and Apoptosis of Nucleus Pulposus Cells through Directly Targeting FOXO1 in Intervertebral Disc Degeneration

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xingyu ChaiGang Zhao

Abstract

microRNA-486-5p (miR-486-5p) and forkhead box protein O1 (FOXO1) play an important role in the development of intervertebral disc degeneration (IDD). However, their molecular mechanisms in IDD remain unknown. qRT-PCR assay was used to identify miR-486-5p expression in nucleus pulposus (NP) cells. In-vitro transfection, CCK-8, flow cytometry and luciferase reporter assay were used to validate the role and relationship of miR-486-5p and FOXO1 in lipopolysaccharides (LPS)-stimulated NP cells. qRT-PCR and Western blot were used to measure the expression levels of inflammatory cytokines, matrix degrading enzymes, and extracellular matrix (ECM)-related genes. miR-486-5p expression was significantly down-regulated, while FOXO1 expression was up-regulated in LPS-treated NP cells (P<0.001). miR-486-5p over-expression repressed LPS-induced expressions of inflammatory cytokines (IL-1β, IL-6 and TNF-α) and matrix degrading enzymes (MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5), and promoted the expressions of LPS-inhibited ECM-related genes (Aggrecan and Collagen II) (all P<0.001). In addition, miR-486-5p over-expression protected NP cells against LPS-induced apoptosis. However, inhibition of miR-486-5p led to the opposite effects. Mechanically, F...Continue Reading

Citations

Jun 25, 2020·Human Cell·Xiaoxia YangZongting Shi
Oct 1, 2020·International Journal of Molecular Sciences·Nadezda PetejovaDavid Stejskal
Jan 18, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Nader Akbari DilmaghnaiSoudeh Ghafouri-Fard
Dec 18, 2020·Mechanisms of Ageing and Development·Hua ZhongChenjun Tian
Nov 28, 2020·International Journal of Chronic Obstructive Pulmonary Disease·Jie ZhangYufeng Wan
Feb 20, 2021·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Hui YeYunlong Zhuang
May 1, 2021·International Journal of Molecular Sciences·Cleonardo Augusto SilvaSidney Santos
Jun 29, 2021·Experimental and Therapeutic Medicine·Fengguang YangQingshan Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis