Abstract
Environmental exposure to particulate matter 2.5 (PM2.5) threatens public health, which has caused worldwide concerns. MicroRNAs (miRNAs, miRs) participate in multiple biological regulation. Among them, miR-486 has been reported to be a beneficial molecule for cell survival in various cell types. However, the potential function of miR-486 in PM2.5-induced cytotoxic is still uncertain. The expression of miR-486 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) after A549 cells incubated with PM2.5. Then TUNEL staining and DCFH-DA fluorescence were used to test the apoptosis and ROS generation of A549 cells after exposed to PM2.5 with miR-486 mimic. Western blot was performed to determine the expression of Bax/Bcl2 ratio. In addition, western blot and rescue experiments were conducted to determine the target gene of miR-486. After treated with PM2.5, the expression of miR-486 was decreased. And miR-486 mimic treatment reduced cell apoptosis and reactive oxygen species (ROS) generation induced by PM2.5 exposure. Further studies showed that miR-486 negatively regulated the protein levels of PTEN and FOXO1. Rescue experiments demonstrated that PTEN and FOXO1 mediated the protective effects of miR-486 in PM2....Continue Reading
Citations
Dec 18, 2019·Environmental Science and Pollution Research International·Cai ChenLeilei Dong
Jun 25, 2020·International Journal of Molecular Sciences·Montserrat ClimentAlessandra Castaldi
Sep 27, 2020·Journal of Endocrinological Investigation·W LiG Liang
Jan 25, 2020·BioMed Research International·Jia HeZhaohui Sun
Nov 1, 2020·Allergy·Julie WeidnerMadeleine Rådinger
Oct 17, 2020·Environmental Pollution·Ken-Ichiro TanakaMasahiro Kawahara
Feb 7, 2021·Journal of Personalized Medicine·Rakhmetkazhy BersimbaevOralbek Ilderbayev
Feb 8, 2021·European Journal of Pharmacology·Wen ChengZhishan Ding
Dec 10, 2020·American Journal of Physiology. Cell Physiology·Chunjian ZhangJiaxin Zheng
Apr 24, 2021·Environmental Research·Álvaro Del RealM Teresa Zarrabeitia
May 3, 2021·Environmental Pollution·Lin XuDianke Yu
Jun 6, 2020·Journal of the American College of Cardiology·Salvatore Patanè
Jun 25, 2021·Open Medicine·Xuan JinMingzhu Yang
Jul 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chunyuan WangLaiyu Song
Aug 14, 2021·Ecotoxicology and Environmental Safety·Mingyang GuanYing Zhang
Aug 17, 2021·Journal of Hazardous Materials·Caixia GuoYanbo Li
Oct 7, 2021·Inflammation·Quan ChenDongmei Zhang
Nov 19, 2021·Biochemical Genetics·Hao WuHaichao Xu
Nov 25, 2021·Advanced Science·Rui ZhengZhengdong Zhang
Dec 3, 2020·Archives of Biochemistry and Biophysics·Yunfei WuXu Wu