miR-486 inhibits PM2.5-induced apoptosis and oxidative stress in human lung alveolar epithelial A549 cells

Annals of Translational Medicine
Jin LiZheng Jiao

Abstract

Environmental exposure to particulate matter 2.5 (PM2.5) threatens public health, which has caused worldwide concerns. MicroRNAs (miRNAs, miRs) participate in multiple biological regulation. Among them, miR-486 has been reported to be a beneficial molecule for cell survival in various cell types. However, the potential function of miR-486 in PM2.5-induced cytotoxic is still uncertain. The expression of miR-486 was detected by quantitative real-time polymerase chain reaction (qRT-PCR) after A549 cells incubated with PM2.5. Then TUNEL staining and DCFH-DA fluorescence were used to test the apoptosis and ROS generation of A549 cells after exposed to PM2.5 with miR-486 mimic. Western blot was performed to determine the expression of Bax/Bcl2 ratio. In addition, western blot and rescue experiments were conducted to determine the target gene of miR-486. After treated with PM2.5, the expression of miR-486 was decreased. And miR-486 mimic treatment reduced cell apoptosis and reactive oxygen species (ROS) generation induced by PM2.5 exposure. Further studies showed that miR-486 negatively regulated the protein levels of PTEN and FOXO1. Rescue experiments demonstrated that PTEN and FOXO1 mediated the protective effects of miR-486 in PM2....Continue Reading

Citations

Jun 25, 2020·International Journal of Molecular Sciences·Montserrat ClimentAlessandra Castaldi
Nov 1, 2020·Allergy·Julie WeidnerMadeleine Rådinger
Feb 7, 2021·Journal of Personalized Medicine·Rakhmetkazhy BersimbaevOralbek Ilderbayev
Dec 10, 2020·American Journal of Physiology. Cell Physiology·Chunjian ZhangJiaxin Zheng
Apr 24, 2021·Environmental Research·Álvaro Del RealM Teresa Zarrabeitia
Jun 6, 2020·Journal of the American College of Cardiology·Salvatore Patanè
Jul 17, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chunyuan WangLaiyu Song
Aug 14, 2021·Ecotoxicology and Environmental Safety·Mingyang GuanYing Zhang
Nov 19, 2021·Biochemical Genetics·Hao WuHaichao Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis