MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Journal of Bioscience and Bioengineering
Yuki IwawakiKatsuhiko Yoshimoto

Abstract

Mechanical stimuli regulate fundamental cell processes such as proliferation, differentiation, and morphogenesis. We attempted to identify microRNA (miRNA) whose expression is changed during compressive treatment in MC3T3-E1, a pre-osteoblastic cell line. Microarray analysis followed by reverse transcription-quantitative polymerase chain reaction revealed that compressive force at 294 Pa for 24 h in MC3T3-E1 cells increased levels of miR-494-3p, miR-146a-5p, miR-210-3p, and miR-1247-3p. Among these miRNAs, miR-494-3p was found to inhibit cell proliferation in MC3T3-E1 cells. Furthermore, cells subjected to compressive force showed slower cell growth compared with control cells. Levels of mRNA for fibroblast growth factor receptor 2 (FGFR2) and Rho-associated coiled-coil kinase 1 (ROCK1), which were predicted to be targets of miR-494-3p, were decreased by compressive force or overexpression of miR-494-3p mimics in MC3T3-E1 cells. Furthermore, binding sites of miR-494-3p within 3'-untranslated regions of Fgfr2 and Rock1 were determined using luciferase reporter assay. In conclusion, compressive force affected expressions of several miRNAs including miR-494-3p in MC3T3-E1 cells. Compressive force might inhibit cell proliferation i...Continue Reading

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Citations

Nov 20, 2016·Journal of Cellular Physiology·Yu YuanJun Zou
Aug 3, 2017·International Journal of Molecular Sciences·Zhihao ChenAirong Qian
May 31, 2018·Bioscience Reports·Yue WangWeiran Li
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Jan 24, 2020·Scientific Reports·Ayşe KılıçScott T Weiss
Aug 11, 2021·Experimental and Therapeutic Medicine·Huan YangYong Guo

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