MiR-494 acts as a tumor promoter by targeting CASP2 in non-small cell lung cancer

Scientific Reports
Qiao ZhangChangzhi Huang

Abstract

MiR-494 plays an important role in several types of human cancers, including non-small cell lung cancer (NSCLC). Although the role of miR-494 has been investigated in several studies, the expression profile and underlying mechanism are still poorly understood. In this study, we found that overexpression of miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis. By using microarray and Dual luciferase reporter assays, we further showed that caspase-2 (CASP2) is a functional target of miR-494, and the expression of CASP2 is inversely associated with miR-494 in vitro. In addition, miR-494 promoted the proliferation and colony formation of NSCLC cells and reduced their sensitivity to cisplatin-induced apoptosis by targeting CASP2. Therefore, our results suggest that miR-494 plays an oncomiR role in NSCLC cells and may be a candidate biomarker for malignant transformation and a therapeutic target of NSCLC.

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Citations

Dec 11, 2019·Technology in Cancer Research & Treatment·Jian-Hua GeChun-Lai Zhang
Jan 21, 2021·OncoTargets and Therapy·Lu RenJun Tian
Nov 26, 2020·International Journal of Molecular Sciences·Young-Ho Ahn, Yoon Ho Ko
May 29, 2021·MicroRNA·Younes El FouniniFadila Guessous

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Methods Mentioned

BETA
surgical resection
transfection
FACS
flow cytometry
PCR
Assay

Software Mentioned

FACS
GraphPad
TargetScan
GraphPad Prism

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