MiR-494 alleviates lipopolysaccharide (LPS)-induced autophagy and apoptosis in PC-12 cells by targeting IL-13

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
Wei Geng, Lei Liu

Abstract

MiR-494 is reported to act as a tumor-suppressive factor that inhibits the proliferation and colony formation of some cancer cells. However, there is still no report available on miR-494 functions in spinal cord injury (SCI) until now. The objective of this study was to examine the status of miR-494 in PC-12 cells injury induced by lipopolysaccharide (LPS), as well as its mechanism. The cell counting kit-8 (CKK-8) assay and apoptosis assay were respectively used to determine the proliferation and apoptosis of PC-12. The reverse transcription polymerase chain reaction (RT-PCR) analysis and western blot analysis displayed the expression of related factors at mRNA and protein level, respectively. The results showed that LPS could significantly decrease cell viability, and promote the cell apoptosis and autophagy of PC-12 in a dose-dependent manner (p < 0.05). The overexpression of miR-494 could protect PC-12 cells from LPS-induced injury, as miR-494 overexpression increased cell viability, and reduced cell apoptosis and autophagy (p < 0.05). MiR-494 played a negative regulatory role in interleukin (IL)-13, and IL-13 was a direct target of miR-494. The overexpression of IL-13 could significantly aggravate LPS-diminished cell viabil...Continue Reading

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