miR-501 is upregulated in cervical cancer and promotes cell proliferation, migration and invasion by targeting CYLD

Chemico-biological Interactions
Jaceline Gislaine Pires SanchesBo Song

Abstract

Cervical cancer is the common gynecological deadly malignancy worldwide. Here we attempted to evaluate the effects and mechanisms of microRNA-501-5p (miR-501) on the cell proliferation, migration, invasion and the clinical significance in the cervical cancer. Cervical cancer HeLa cells were transfected with miR-501 mimic or inhibitor or siRNA against Cylindromatosis (CYLD) using Lipofectamine 2000. miR-501 expression was assessed in HeLa cells and cervical cancer specimens by real-time qRT-PCR. The functional roles of miR-501 were determined by CCK-8, colony formation, scratch wound healing and transwell assays. The apoptosis rate was detected by flow cytometry assay. CYLD, BCL-2, BAX, NF-κB p65 and phosphorylated p65 (p-p65) proteins were examined by Western blotting. CYLD expression was evaluated by immunohistochemistry in cervical cancer tissues. miR-501 was upregulated, whereas CYLD protein was downregulated in cervical cancer tissues compared to normal cervical tissues. miR-501 overexpression and CYLD protein downregulation were positively correlated with poor differentiation, tumor size, International Federation of Gynecology and Obstetrics (FIGO) stage and lymph node metastasis. CYLD was downregulated by miR-501 at both ...Continue Reading

Citations

Dec 20, 2018·Genome Research·Maura L GillisonDavid E Symer
Mar 6, 2019·Bioscience Reports·Jin-Yan Wang, Li-Juan Chen
May 22, 2019·International Journal of Clinical Oncology·Congwei HuangChengzhe Jin
Jul 19, 2019·Experimental and Therapeutic Medicine·Yu SunKun Han
Sep 18, 2018·Molecular Medicine Reports·Chuan-Gang ZhangXia Wu
Aug 1, 2019·International Journal of Oncology·Fangxiong WuFengrui Liu
Aug 27, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Ji ChengWenyi Wei
Feb 7, 2021·Experimental Gerontology·Sira KarvinenEija K Laakkonen
Nov 6, 2020·Cancer Management and Research·Yanling LinLongmei Cai
Apr 4, 2021·Cancers·Shailendra Kumar Dhar DwivediResham Bhattacharya
May 10, 2021·Journal of Gynecology Obstetrics and Human Reproduction·Mohammad AbbasFarzana Mahdi
May 24, 2021·Cellular & Molecular Biology Letters·Xiaomei SunBeihua Kong

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