miR-503-5p inhibits colon cancer tumorigenesis, angiogenesis, and lymphangiogenesis by directly downregulating VEGF-A.

Gene Therapy
Linlin WeiShichen Sun

Abstract

MicroRNAs (miRNAs) are considered important in the pathogenesis of colon cancer. But the mechanism of their role in colon cancer is still largely unknown. Here, we aimed to explore the function of miR-503-5p in the pathogenesis of colon cancer. This study analyzed miRNA microarray of colon cancer. Then, we performed EdU, CCK-8, flow cytometry, Transwell invasion assays and in vivo assays to explore the exact role of miR-503-5p in colon cancer. We observed considerable downregulation of miR-503-5p expression in colon cancer cells and tissues and significant correlation with the TNM stage, differentiation grade and lymph node metastasis of colon cancer. Overexpression of miR-503-5p promoted the apoptosis and G1 arrest of colon cancer cells, and inhibited migration, proliferation, invasion and colony formation. Interestingly, ectopic miR-503-5p overexpression could significantly inhibit vascular endothelial growth factor (VEGF)-A expression and reduce the activity of a luciferase reporter containing the VEGF-A 3'-untranslated region. Furthermore, overexpressed miR-503-5p in human umbilical vein endothelial cells (HUVECs) and colon cancer cells resulted in lower expression levels of VEGFR-2, and subsequently inhibited AKT signaling...Continue Reading

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Citations

Nov 12, 2020·Cancers·Khairunnisa' Md YusofKelly A Avery-Kiejda
Jun 8, 2021·Molecular Therapy Oncolytics·Zahra Sadat RazaviHamed Mirzaei
Aug 9, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Behnia AkbariHamid Reza Mirzaei
Sep 7, 2021·Medicinal Research Reviews·Sara RezzolaRoberto Ronca

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