MiR-506 is down-regulated in clear cell renal cell carcinoma and inhibits cell growth and metastasis via targeting FLOT1

PloS One
Feng-qiang YangJun-hua Zheng

Abstract

Some microRNAs (miRNAs) are abnormally expressed in cancer and contribute to tumorigenesis. In the present study, we investigated the role of miR-506 in clear cell renal cell carcinoma (ccRCC). miR-506 expression was detected in renal cancer cell lines 786-O, ACHN, Caki-1, and Caki-2 and ccRCC specimens by quantitative real-time-PCR. We assessed the association of miR-506 expression with pathology and prognosis in ccRCC patients. We over-expressed and knocked-down miR-506 expression in two renal cancer cell lines, 786-O and ACHN, and assessed the impact on cell proliferation, migration and invasion. A luciferase reporter assay was conducted to confirm the target gene of miR-506 in renal cancer cell lines. miR-506 was significantly down-regulated in renal cancer cell lines and ccRCC specimens. Low miR-506 expression in ccRCC specimens was associated with an advanced clinical stage and poor prognosis. miR-506 expression was an independent prognostic marker of overall ccRCC patient survival in a multivariate analysis. Over-expression of miR-506 in renal cancer cells decreased cell growth and metastasis, In contrast, down-regulation of miR-506 expression promoted renal cancer cell growth and metastasis. FLOT1, a potential target ge...Continue Reading

References

Jun 21, 2005·Nature Genetics·Isaac BentwichZvi Bentwich
Oct 28, 2005·The New England Journal of Medicine·Chang-Zheng Chen
Apr 10, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Pierre I KarakiewiczJean-Jacques Patard
May 8, 2007·European Journal of Cell Biology·Tanja Babuke, Ritva Tikkanen
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Zhong JiangChin-Lee Wu
Mar 5, 2009·Journal of Cell Science·Kirsi RientoBenjamin J Nichols
Dec 23, 2009·The International Journal of Biochemistry & Cell Biology·William C S Cho
Jan 9, 2010·Pathology International·Hiroshi MiyamotoJonathan I Epstein
Mar 31, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chuyong LinLibing Song
Jun 28, 2011·Analytica Chimica Acta·Mariàngels de Planell-Saguer, María Celina Rodicio
Mar 3, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jesús M BanalesNicholas F LaRusso
Apr 12, 2012·Carcinogenesis·Marilena V Iorio, Carlo M Croce
Oct 20, 2012·Current Opinion in Oncology·Ramiro Garzon, Guido Marcucci
May 30, 2013·PloS One·Himanshu AroraWoong-Yang Park
Nov 28, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui LiXing-Guo Zhang

❮ Previous
Next ❯

Citations

Aug 5, 2015·Molecular Medicine Reports·Jundong DuHuabo Jiao
Oct 8, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hai-Ting Liu, Peng Gao
Nov 29, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun-Jie ZhangChen-Yu Wang
Feb 17, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jingmei ZhongXiong-Jian Luo
Oct 4, 2017·Oncotarget·Meijun DuLiang Wang
Aug 20, 2016·Oncotarget·Jian LiHuaizhi Wang
Oct 21, 2015·The International Journal of Biological Markers·Michal FedorkoMohamed I Nussir
Apr 17, 2020·Cancer Metastasis Reviews·Cécile Gauthier-RouvièreDamien Planchon
Oct 16, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xiaofang WangChenguang Zhang
Nov 12, 2015·Oncology Reports·Tao PengYongxin Luan
Sep 5, 2020·Cancer Management and Research·Xiaolei ZhaoJunqing Hou
May 16, 2020·Frontiers in Neurology·Dahao Wu, Changzhen Wang
Oct 2, 2019·Experimental and Therapeutic Medicine·Xiaogang Yang, Baojun Yang
Oct 14, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaotong SuHaichao Liu
Aug 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dapeng WuXuejian Wu
Oct 1, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei YangShiyang Zhang
Mar 27, 2021·Journal of Experimental & Clinical Cancer Research : CR·Kosuke YoshidaHiroaki Kajiyama

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
Assay
flow cytometry
protein assay

Software Mentioned

miRanda
SPSS
TargetScan

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Adenocarcinoma, Clear Cell

Clear Cell Adenocarcinoma is a tumor that arises in the female genital tract and is characterized by cells that appear clear under the microscope. Discover the latest research here.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis