MiR-513b-5p represses autophagy during the malignant progression of hepatocellular carcinoma by targeting PIK3R3.

Aging
Wei JinRongjun Nie

Abstract

Hepatocellular carcinoma (HCC) serves as a prevailing global malignancy with severe mortality and extremely unsatisfactory prognosis, in which autophagy is a fundamental process in liver cancer pathogenesis, but the mechanisms are poorly understood. MicroRNAs (miRNAs) serve as a type of well-recognized non-coding regulators and contribute to the modulation of liver cancer development, from the aspects of diagnosis, progression, and therapy. Here, we aimed to investigate the function of hsa_microRNA-513b-5p (miR-513b-5p) in regulating autophagy during HCC progression. Specifically, our data showed that miR-513b-5p mimic reduced the LC3-II and beclin1 expression but enhanced p62 expression in HCC cells. MiR-513b-5p repressed liver cancer cell proliferation, migration/invasion, and induced apoptosis in vitro. Crucially, miR-513b-5p attenuated tumor growth of liver cancer cells in vivo. In the mechanical investigation, we identified that PIK3R3 mRNA 3'UTR was targeted by miR-513b-5p and miR-513b-5p suppressed PIK3R3 expression. PIK3R3 overexpression partly reversed miR-513b-5p-mediated autophagy, proliferation, and apoptosis of liver cancer cells. Consequently, we concluded that miR-513b-5p repressed autophagy during the malignant ...Continue Reading

References

Jan 23, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gang CaoShiyuan Liu
Apr 26, 2017·Journal of Hepatology·Marcel KlingenbergTushar Patel
May 26, 2018·Biological Reviews of the Cambridge Philosophical Society·Shama P KabekkoduKapaettu Satyamoorthy
Nov 25, 2018·Journal of Cellular Physiology·Kioomars SaliminejadSeyed Hamidollah Ghaffari
Mar 2, 2019·Nucleic Acids Research·Julia AllesEckart Meese
Oct 31, 2019·Trends in Pharmacological Sciences·Qianjin LuEsteban Ballestar
Jan 30, 2021·Nature Reviews. Gastroenterology & Hepatology·Josep M LlovetRiccardo Lencioni
Jan 31, 2021·Journal of Hepatology·Laura Conde de la RosaJose C Fernandez-Checa
Feb 5, 2021·Theranostics·Xiaomeng DaiWeijia Fang

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