MiR-514 attenuates proliferation and increases chemoresistance by targeting ATP binding cassette subfamily in ovarian cancer

Molecular Genetics and Genomics : MGG
Sha XiaoDeling Wang

Abstract

Cisplatin is one of the most popular chemotherapeutic drugs in treating ovarian cancer. Resistance to cisplatin is a common clinical challenge that needs to be solved to increase its anti-tumor effects. The relation of miR-514 expression with prognosis in ovarian cancer patients was analyzed based on GSE73584 datasets. The regulation of miR-514 on proliferation and cisplatin chemosensitivity of ovarian cells was examined by MTT assay, colony-formation assay and soft-agar colony-formation assay. Dual luciferase assay was performed to detect the direct interaction of miR-514 with its downstream targets. Immunobloting and qRT-PCR were performed for target gene expression analysis. Low expression of miR-514 was related to poor prognosis in ovarian cancer patients. MiR-514 repressed proliferation and decreased cisplatin chemosensitivity in ovarian cancer cells by targeting ATP binding cassette subfamily. MiR-514 is of clinically significance in ovarian cancer by attenuating proliferation of ovarian cancer cells and decreasing chemoresistance of cisplatin by targeting ATP binding cassette subfamily.

References

Feb 6, 2003·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 25, 2005·Current Drug Delivery·Hristos GlavinasBalázs Sarkadi
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
Feb 19, 2009·Journal of Mammary Gland Biology and Neoplasia·Michael Dean
Nov 28, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Zofia WotschofskyKlaus Jung
Oct 5, 2013·Inflammation & Allergy Drug Targets·Christa BuechlerSabrina Krautbauer
Jun 13, 2015·Journal of Visualized Experiments : JoVE·Sachi HoribataScott A Coonrod
Jan 24, 2017·Molecular Carcinogenesis·Lingjie BaoXiaofang Yi
Jan 13, 2018·International Journal of Molecular Medicine·Chen LinJianhua Chang
Jan 13, 2018·International Journal of Molecular Medicine·Jiao LiuChuize Kong
Jan 14, 2018·Gynecologic Oncology·Ghassan M SaedIra Memaj

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Citations

Mar 4, 2020·Molecular Cancer·Xinyi ZhangYongguang Tao
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ce XingFeng Bai
Aug 5, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yi XuYunfu Cui
Apr 20, 2021·Frontiers in Immunology·Xueqin ZouQixiang Shao
Oct 2, 2021·Journal of Ovarian Research·Meysam Moghbeli

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Datasets Mentioned

BETA
GSE73584

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

targetcan

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