MiR-519d suppresses breast cancer tumorigenesis and metastasis via targeting MMP3

International Journal of Biological Sciences
Chengling ChuYong Zhang

Abstract

Breast cancer (BC) is the most common cause of death in women throughout the world. Although microRNAs (miRNAs) have been identified as novel regulators in carcinogenesis, there are still abundant hidden treasure needed to be excavated. In the present study, we found that miR-519d expression was remarkably decreased in both human BC tissues and MCF-7 cells. CCK8 and 5-Ethynyl-2'-deoxyuridine (EdU) assays were used to evaluate cell proliferation. Wound-healing and transwell assays were performed for detection of cell migration and invasion. The results demonstrated miR-519d overexpression dramatically suppressed MCF-7 cells proliferation, migration and invasion. While downregulation of miR-519d by miR-519d inhibitor substantially increased MCF-7 cell carcinogenesis. Further analysis identified Matrix Metalloproteinase-3 (MMP3) as a direct target of miR-519d. QRT-PCR and western blot results indicated the correlative expression of miR-519d and MMP3 in BC tissues and MCF-7 cells. In summary, our data uncovered the novel molecular interaction between miR-519d and MMP3, indicating a therapeutic strategy of miR-519d for BC.

Citations

Oct 30, 2019·Bioscience, Biotechnology, and Biochemistry·Lishan Ma, Jin Li
Feb 6, 2019·International Journal of Oncology·Ping ChenZhenling Ji
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Oct 11, 2019·Cells·Mohammad Mijanur RahmanTrygve O Tollefsbol
May 24, 2019·Fundamental & Clinical Pharmacology·Ying CuiYuanfei Yao
Nov 11, 2020·International Journal of Molecular Sciences·Sylwia NakoniecznaWirginia Kukula-Koch
Dec 31, 2020·OncoTargets and Therapy·Haiming MaXiaokang Liu
Feb 20, 2021·Experimental Cell Research·Madhumathy G NairT S Sridhar
Aug 28, 2021·International Journal of Molecular Sciences·Angela Ximena Argote CamachoMaría Isabel Núñez
Sep 9, 2021·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Pihong LiMingdong Lu

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Datasets Mentioned

BETA
MDA-MB-231

Methods Mentioned

BETA
biopsies
transfection
PCR
Protein Assay
electrophoresis
Infrared Imaging
confocal microscopy

Software Mentioned

TargetScan

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