MiR-520b/e Regulates Proliferation and Migration by Simultaneously Targeting EGFR in Gastric Cancer

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Shuang LiYi Ba

Abstract

MicroRNAs (miRNAs) have been demonstrated to play a crucial role in tumorigenesis. Previous studies have shown that miR-520b/e acts as a tumor suppressor in several tumors. Other studies indicated that epidermal growth factor receptor (EGFR) is highly expressed in many tumors, and involved in the development of tumors, such as cell proliferation, migration, angiogenesis and apoptosis. However, the correlation of miRNAs and EGFR in gastric cancer (GC) has not been adequately investigated. Our aim was to explore the relationship. The expression levels of EGFR and miR-520b/e were examined by RT-PCR and Western blot. We also investigated the relationship between EGFR and miR-520b/e in GC cell lines by relevant experiments. In this study, we found that miR-520b/e inhibits the protein expression of EGFR by directly binding with the 3'-untranslated region (3'-UTR). And it was shown that the down-regulation of miR-520b/e promotes cell proliferation and migration by negative regulation of the EGFR pathway, while over-expression of miR-520b/e inhibits these properties. In addition, the biological function of EGFR in GC cell lines was validated by silencing and over-expression assays respectively. Taken together, our results demonstrate t...Continue Reading

Citations

Aug 15, 2018·Thoracic Cancer·Ke JinHuizhong Zhang
Nov 30, 2018·Cell Proliferation·Rong WangYongqian Shu
May 12, 2019·Journal of Clinical Medicine·Vitalba RuggieriSimona Laurino
Jun 9, 2020·Environmental Toxicology·Zhaohui FanPudong Qian
Nov 8, 2018·Experimental and Therapeutic Medicine·Linlin Zhang, Shuangquan Yu
Mar 14, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Jing LiuGuo-Jun Zhang
Aug 22, 2020·Cancer Cell International·Wendao YouRui Li

Related Concepts

EGFR protein, human
MicroRNA-520b
DNA Sequence
Cell Motility
Luciferases
Hereditary Diffuse Gastric Cancer
Receptor Down-Regulation
Receptor Up-Regulation
Gene Expression Regulation, Neoplastic
Genes, Reporter

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.