miR-539 induces cell cycle arrest in nasopharyngeal carcinoma by targeting cyclin-dependent kinase 4

Cell Biochemistry and Function
Ling-yan LvXing-jie Wang

Abstract

Dysregulation of microRNAs has been demonstrated to contribute to malignant progression of cancers, including nasopharyngeal carcinoma (NPC). miR-539 was previously reported to be significantly downregulated in osteosarcoma. However, the potential role and mechanism of action of miR-539 in the initiation and progression of NPC remain largely unknown. Quantitative reverse transcription (RT)-PCR demonstrated that miR-539 was significantly downregulated in NPC tumour tissues compared with nontumour tissues. The cell viability, colony formation assay and tumourigenicity assays in nude mice showed that miR-539 could inhibit NPC cell growth in vitro and in vivo. The cyclin-dependent kinase 4 (CDK4) was verified as a miR-539 target gene using dual-luciferase reporter assays, quantitative RT-PCR and Western blotting and was involved in miR-539-regulated NPC cell growth. These results indicated that miR-539 plays an important role in the initiation and progression of NPC by targeting CDK4 and the miR-539/CDK4 pathway may contribute to the development of novel therapeutic strategies for NPC in the future.

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Citations

Dec 22, 2017·Journal of Cellular Biochemistry·Xianzheng GaoMingzhi Zhang
Nov 5, 2019·Current Molecular Pharmacology·Ting ZhangWeiguo Huang
Nov 20, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Fenglin CaiJinhai Tang
Dec 26, 2018·Journal of Cellular Biochemistry·Guoqiang QiShuaiqun Wang
Sep 25, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dacheng WenShutao Zhao
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Huixing DengYang Aimin
Apr 26, 2019·Experimental and Molecular Pathology·Haibo YuBujian Pan
Jul 14, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Junjie QuanLe Zhou

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