miR-551b regulates epithelial-mesenchymal transition and metastasis of gastric cancer by inhibiting ERBB4 expression

Guangyuan SongZaiyuan Ye


Epithelial-mesenchymal transition (EMT) is an important biological process that is characteristic of malignant tumor cells with metastatic potential. We investigated the role of miR-551b in EMT and metastasis in gastric cancer (GC). We found that low miR-551b levels were associated with EMT, metastasis and a poor prognosis in GC patients. Further, two GC cell lines, MNK45 and SGC7901, exhibited lower miR-551b levels than the GES normal stomach cell line. Exposing MNK45 and SGC7901 cells to TGF-β1 resulted in cell morphology changes characteristic of EMT, which was confirmed by Western blot analysis demonstrating low E-Cadherin and high N-Cadherin and Vimentin levels. Treatment with miR-551b mimics inhibited these EMT changes as well as Transwell migration and invasiveness. We identified ERBB4 as a potential target of miR-551b based on patient data from the TCGA. ERBB4 was upregulated in GC specimens, and its high expression correlated with a poor prognosis of GC patients. Dual luciferase assays revealed that miR-551b directly inhibited ERBB4 by binding to its 3'UTR. Moreover, treatment with miR-551b mimics or the ERBB4 inhibitor AST-1306 inhibited EMT in the GC cell lines. Finally, nude mice xenografted with GC cancer cell line...Continue Reading


Jun 15, 2019·Journal of Cellular and Molecular Medicine·Weiping ChangZhimin Geng
Mar 29, 2020·Cellular Oncology (Dordrecht)·Vincent F M SegersGilles W De Keulenaer


Jul 19, 2003·Science·James C Carrington, Victor Ambros
Mar 29, 2008·Science·Oliver Hobert
Jan 21, 2009·Cell Research·Jian XuRik Derynck
Feb 4, 2010·Genome Biology·Ashley P E Roberts, Catherine L Jopling
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Jul 12, 2011·Physiological Reviews·Danish Sayed, Maha Abdellatif
Nov 16, 2011·Seminars in Oncology·Francesca LovatCarlo M Croce
Jun 2, 2012·The Lancet Oncology·Yi W KongMartin Bushell
Nov 21, 2012·Nature Reviews. Gastroenterology & Hepatology·Shumei Song, Jaffer A Ajani
Jan 15, 2013·Current Opinion in Cell Biology·Matthew T Blahna, Akiko Hata
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Aug 3, 2013·Nature Reviews. Immunology·Dirk Baumjohann, K Mark Ansel
Oct 2, 2013·Annual Review of Pathology·Gianpiero Di LevaCarlo M Croce
Nov 1, 2013·Nature Reviews. Drug Discovery·Hui LingGeorge A Calin
Mar 3, 2015·The Journal of Clinical Investigation·Matjaz RokavecHeiko Hermeking
Oct 3, 2015·CA: a Cancer Journal for Clinicians·Elizabeth M WardTed Gansler
Jul 20, 2016·Journal of Clinical Medicine·Joema Felipe LimaJohn M S Bartlett
Nov 22, 2016·Nature Genetics·Ross A OkimotoTrever G Bivona

Related Concepts

Gene Expression Regulation, Neoplastic
The Cancer Genome Atlas
VIM gene
Untranslated Regions
TNM Staging System
Serum Vitamin D Measurement
ERBB4 gene
SLC14A2 gene
Malignant Neoplasm of Stomach

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