miR-5591-5p regulates the effect of ADSCs in repairing diabetic wound via targeting AGEs/AGER/JNK signaling axis

Cell Death & Disease
Qiang LiPeisheng Jin

Abstract

Advanced glycation end products/advanced glycation end products receptor (AGEs/AGER) interaction triggers reactive oxygen species (ROS) generation and activates downstream signal pathways and induces apoptosis in endothelial progenitor cells. A number of studies have revealed the involvement of microRNAs (miRNAs) in regulating intracellular ROS production and apoptosis. However, few studies explore the role of miRNAs in regulating the effect of adipose tissue-derived stem cells (ADSCs) in repairing diabetic wound and the associated cellular mechanisms remain unclear. In this study, ADSCs were exposed to AGEs, then siRNA for AGER was transfected into ADSCs. We found that AGEs/AGER axis induced ROS generation and apoptosis in ADSCs. AGEs treatment downregulated miR-5591-5p in ADSCs, which directly targeted AGER. miR-5591-5p suppressed AGEs/AGER axis-mediated ROS generation and apoptosis in ADSCs in vitro. In addition, miR-5591-5p promoted cell survival and enhanced the ability of ADSCs for repairing cutaneous wound in vivo. Furthermore, we confirmed that c-jun kinase (JNK) signal was involved in the inhibitory effect of miR-5591-5p on AGEs/AGER axis-induced ROS generation and apoptosis in ADSCs. Thus, these results indicated that...Continue Reading

References

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Citations

Mar 8, 2019·Aesthetic Plastic Surgery·Wanling ZhengAijun Zhang
Oct 26, 2018·Stem Cell Research & Therapy·Qiang LiPeisheng Jin
Aug 23, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuejun YaoChangyou Gao
Jan 29, 2019·Frontiers in Pharmacology·Qiang LuFenghua Zhou
Oct 4, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Sheldon RowanAllen Taylor
Mar 19, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
May 14, 2021·Molecular Therapy. Nucleic Acids·Jianyi LiChengliang Deng
Feb 1, 2020·The Journal of Investigative Dermatology·Yating YinPeisheng Jin
Aug 28, 2021·Archives of Gerontology and Geriatrics·Lintao LiJingchuan Sun

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Methods Mentioned

BETA
PCR
flow cytometry
transfection
Fluorescence

Software Mentioned

SPSS
targetscan
miRDB

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis