miR-590 regulates WT1 during proliferation of G401 cells

Molecular Medicine Reports
Liyi HongHong Zhu


Nephroblastoma (Wilms' tumor) is frequently associated with mortality in children. MicroRNAs (miRNAs) are important for tumor development serving as oncogenes or tumor suppressors. In the present study, miRNA‑590 (miR‑590) was identified to be upregulated in Wilms' tumor tissues compared with the normal adjacent tissues. Additionally, the levels of miR‑590 were consistent with their clinical stage. Wilms' tumor 1 (WT1) was considered to be a tumor suppressor in certain tumor types, and it has been detected at low expression levels in various types of cancer with high cell proliferation and aggressive behavior. The expression levels of miR‑590 were quantified using reverse transcription‑quantitative polymerase chain reaction. Cell proliferation was measured using 5‑ethynyl‑20‑deoxyuridine assays. The protein expression levels of WT1 were investigated by western blot analysis. To the best of our knowledge, the present study was the first to determine that WT1 was a target gene of miR‑590 as miR‑590 was able to negatively regulate WT1 expression level by binding to the specific target site within the 3'‑untranslated region (3'‑UTR) of WT1 in G401 cells. Additionally, overexpression of miR‑590 promoted G401 cell proliferation which...Continue Reading


Jan 1, 1990·Pediatric Pathology·J B BeckwithJ F Bonadio
Jun 1, 1993·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M F TournadeD Bürger
Apr 2, 1998·International Review of Cytology·A L MenkeA G Jochemsen
Oct 22, 1998·American Journal of Medical Genetics·J B Beckwith
Sep 7, 2002·International Journal of Hematology·Hans D MenssenEckhard Thiel
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoshihiro OkaHaruo Sugiyama
Oct 27, 2004·Cancer Science·Yusuke OjiHaruo Sugiyama
Nov 13, 2004·International Journal of Cancer. Journal International Du Cancer·Saeid Amini NikSabine Tejpar
Sep 22, 2006·Human Molecular Genetics·Peter Hohenstein, Nicholas D Hastie
Apr 7, 2007·Nature Clinical Practice. Urology·Ghislaine Scélo, Paul Brennan
Oct 1, 2007·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Hemant B TongaonkarThyavihalli B Yuvaraja
Jul 8, 2010·International Journal of Cancer. Journal International Du Cancer·Joachim SchüzKjeld Schmiegelow
Jul 31, 2010·Paediatric and Perinatal Epidemiology·Anna ChuRayjean J Hung
Mar 23, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Sanjeev Akkina, Bryan N Becker
Jun 12, 2012·Cancer Metastasis Reviews·Jinsong Zhang, Li Ma
Jul 17, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Norbert GrafJan de Kraker
Aug 14, 2012·World Journal of Pediatrics : WJP·Ochicha OchichaDalhatu Gwarzo
Apr 20, 2013·International Journal of Molecular Sciences·Xiangxiang ShanFen Zhou
Nov 30, 2013·Journal of Cellular Biochemistry·Yanxia ChuHui Wang
Sep 26, 2015·BMC Research Notes·Sisay Yifru, Dagnachew Muluye
Oct 16, 2015·Molecular Medicine Reports·Tianming LiuYaguang Weng

❮ Previous
Next ❯


Jan 1, 2020·The Kaohsiung Journal of Medical Sciences·Hong LiuHong Ma
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco

❮ Previous
Next ❯

Methods Mentioned


Software Mentioned

GraphPad Prism

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.