MiR-598 Suppresses Invasion and Migration by Negative Regulation of Derlin-1 and Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Fengming YangHua Shen

Abstract

MicroRNAs regulate a wide range of biological processes of non-small cell lung cancer (NSCLC). Although miR-598 has been reported to act as a suppressor in osteosarcoma and colorectal cancer, the physiological function of miR-598 in NSCLC remains unknown. In this study, the role of miR-598 in NSCLC was investigated. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to estimate the expression of miR-598 and Derlin-1 (DERL1) in both NSCLC tissues and cell lines. Immunohistochemistry (IHC) analyzed the association between the miR-598 expression and epithelial-mesenchymal transition (EMT) hallmark genes (E-cadherin, Vimentin) by staining the tumors representative of the high- and low-expression groups. The effect of miR-598 and DERL1 on invasion and migration was determined in vitro using transwell and wound-healing assays. The molecular mechanism underlying the relevance between miR-598 and DERL1 was elucidated by luciferase assay and Western blot. Western blot assessed the expression levels of EMT hallmark genes in cell lines. Xenograft tumor formation assay was conducted as an in vivo experiment. In this study, a relatively low level of miR-598 and high DERL1 expressions were found in NSCLC specimens and c...Continue Reading

Citations

Oct 27, 2018·Journal of Cellular Physiology·Huiling LiangYuanbo Cui
Mar 3, 2019·Journal of Cellular Biochemistry·Li FengYonggang Zhu
Feb 6, 2019·Veterinary and Comparative Oncology·Franziska SchmidBarbara Pratscher
Nov 2, 2019·Cell Biology International·Jinsheng ZengAnwen Liu
Feb 15, 2020·Postgraduate Medical Journal·Chen DaiZhihua Xu
Mar 1, 2019·Cancers·Shang-Gin WuJin-Yuan Shih
Sep 5, 2020·OncoTargets and Therapy·Jiang-Tao PuTian-Yang Dai
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuelong ZhangQi Zhang

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