miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

International Journal of Oncology
Norahayu Othman, Noor Hasima Nagoor

Abstract

Lung cancer remains a major health problem with a low 5-year survival rate of patients. Recent studies have shown that dysregulation of microRNAs (miRNAs) are prevalent in lung cancer and these aberrations play a significant role in the progression of tumour progression. In the present study, bioinformatics analyses was employed to predict potential miR-608 targets, which are associated with signaling pathways involved in cancer. Luciferase reporter assay identified AKT2 as a novel target of miR-608, and suppression of its protein levels was validated through western blot analysis. Zebrafish embryos were microinjected with cells transfected with miR-608 to elucidate the role of miR-608 in vivo, and immunostained with antibodies to detect activated caspase-3. We present the first evidence that miR-608 behaves as a tumour suppressor in A549 and SK-LU-1 cells through the regulation of AKT2, suggesting that selective targeting of AKT2 via miR-608 may be developed as a potential therapeutic strategy for miRNA-based non-small cell lung cancer (NSCLC) therapy.

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Citations

Aug 8, 2018·Chinese Medical Journal·Xiang ZhangLiang-Cheng Zheng
Jul 10, 2019·International Journal of Molecular Sciences·Po-Chun ChenChih-Hsin Tang
Dec 16, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Nasha ZhangMing Yang
May 20, 2018·Biochemical and Biophysical Research Communications·Ling HeLian-Bao Kong
Sep 6, 2018·Biochemical and Biophysical Research Communications·Heling JiaoWei Zhang

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