MiR-615 inhibits cell proliferation, migration and invasion by targeting EGFR in human glioblastoma

Biochemical and Biophysical Research Communications
Yanwei JiHaoran Hu

Abstract

MiR-615 and epidermal growth factor receptor (EGFR) are associated with a number of disease processes and pathogenesis. However, little is known about the mechanisms of miR-615 and EGFR in human glioblastoma multiforme (GBM). Here, we found that down-regulation of miR-615 expression occurred in GBM tissues and cells, and was inversely correlated with overall survival, relapse-free survival, WHO grade as well as EGFR expression. We further determined that miR-615 functions as a tumor suppressor by inhibiting GBM cell proliferation, cell cycle, migration and invasion, and promoting cell apoptosis. In-vivo assay validated the inhibition effect of miR-615 on tumor growth and EGFR expression. Luciferase reporter assays demonstrated that miR-615 targeted the 3'-untranslated region (3'-UTR) of EGFR. Besides, over-expression of EGFR reversed the inhibition effects of miR-615, while silencing of EGFR aggravated these inhibition effects. In conclusions, we identified that miR-615 plays a tumor suppressor role in GBM cell proliferation, migration and invasion by targeting EGFR expression, and miR-615 may act as a novel biomarker for early diagnosis or therapeutic targets of GBM.

Citations

Oct 23, 2019·Journal of Cellular and Molecular Medicine·Katarzyna Pietraszek-GremplewiczDorota Nowak
Jul 2, 2020·Cells·Marisol Godínez-Rubí, Daniel Ortuño-Sahagún
Feb 25, 2020·Journal of Oncology·Adrianna RutkowskaPiotr Rieske
Dec 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bin XuJunfei Shao

Related Concepts

EGFR protein, human
MIRN615 microRNA, human
DNA Sequence
Brain Tumor, Recurrent
Cell Motility
Glioblastoma Multiforme
Neoplasm Invasiveness
Epidermal Growth Factor Receptor
Receptor Down-Regulation
Gene Expression Regulation, Neoplastic

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