MiR-615 Regulates NSC Differentiation In Vitro and Contributes to Spinal Cord Injury Repair by Targeting LINGO-1

Molecular Neurobiology
Hong-Fu WuChuanming Luo


LINGO-1(LRR and Ig domain-containing NOGO receptor interacting protein 1) is a viable target for spinal cord injury (SCI) repair due to its potent negative regulation in neuron survival and axonal regeneration. Although promising, the intracellular mechanism underlying LINGO-1 regulation is unclear. Here, we identified miR-615 as a potential microRNA (miRNA) that directly targets LINGO-1 by binding its 3'-untranslated region (3'-UTR) and caused the translation inhibition of LINGO-1. MiR-615 negatively regulated LINGO-1 during neural stem cell (NSC) differentiation and facilitated its neuronal differentiation in vitro. Interestingly, compared to the control, neurons differentiated from miR-615-treated NSCs were immature with short processes. Further results showed LINGO-1/epidermal growth factor receptor (EGFR) signaling may be involved in this process, as blockade of EGFR using specific antagonist resulted in mature neurons with long processes. Furthermore, intrathecal administration of miR-615 agomir in SCI rats effectively knocked down LINGO-1, increased neuronal survival, enhanced axonal extension and myelination, and improved recovery of hindlimbs motor functions. This work thus uncovers miR-615 as an effective miRNA that r...Continue Reading


Feb 18, 2004·Nature Neuroscience·Sha MiR Blake Pepinsky
May 27, 2006·Molecular Systems Biology·Kanae OdaHiroaki Kitano
Oct 3, 2006·Molecular and Cellular Neurosciences·Benxiu JiJane K Relton
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Haruhisa InoueOle Isacson
Jan 3, 2008·PloS One·Joost M Woltering, Antony J Durston
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Apr 14, 2009·The Journal of Biological Chemistry·Zhaohuan ZhangCheng He
Jul 7, 2009·Experimental Neurology·Nai-Kui LiuXiao-Ming Xu
Sep 19, 2009·Neuromolecular Medicine·Emmette R HutchisonMark P Mattson
Oct 14, 2009·Drug Discovery Today·Reema RoshanBeena Pillai
Mar 29, 2011·Gene Expression Patterns : GEP·Rashmi TripathiAnton J Enright
Jul 21, 2011·Seminars in Cancer Biology·Raymond L StallingsPatrick G Buckley
Jun 16, 2012·Journal of Molecular Neuroscience : MN·Toshio KojimaTsuyomu Ikenoue
Jul 28, 2012·Experimental Neurology·Kartavya SharmaShuxin Li
May 8, 2013·Journal of Neurotrauma·Jian-Zhong HuHong-Bin Lu
Jul 19, 2013·Neuroscience Bulletin·Yi-Min Yuan, Cheng He
Nov 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jing Wang, Robert K Yu
Jun 20, 2014·Nature Protocols·Raju TomerKarl Deisseroth
Apr 23, 2015·Experimental Neurology·Scott M Dyck, Soheila Karimi-Abdolrezaee
Jul 6, 2015·Neuroscience and Biobehavioral Reviews·Jessica L Andrews, Francesca Fernandez-Enright
Apr 2, 2018·Biochemical and Biophysical Research Communications·Yanwei JiHaoran Hu


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Methods Mentioned

electron microscopy

Related Concepts

Epidermal Growth Factor Receptor
Spinal Cord Injuries
Antibody to Myelin Measurement
3' Untranslated Regions
Neural Stem Cells
Neuron Differentiation

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