MiR-665 aggravates heart failure via suppressing CD34-mediated coronary microvessel angiogenesis

Aging
Jiahui FanDao Wen Wang

Abstract

Heart failure (HF) is a major public health problem worldwide. The development of HF was related to coronary microvessel dysfunction. Whether miRNAs participate in HF by regulating coronary microvessel function remain unclear. The potential targets of miR-665 were predicted by rnahybrid software, then verified through anti-Ago2 co-immunoprecipitation, Western blotting and luciferase reporter assays. rAAV9 system was used to manipulate the expression of miR-665 in vivo. Significant increase of miR-665 was observed in endothelial cells of human heart with heart failure. In vitro over-expression of miR-665 in endothelial cells resulted in decreased proliferation but enhanced apoptosis. rAAV-mediated delivery of miR-665 reduced coronary microvessel angiogenesis and cardiac microvessel density, then further impaired cardiac function in vivo. Furthermore, CD34 was confirmed as one of the miR-665 targets. Consistently, re-expression of CD34 attenuated miR-665-mediated damage effects in vitro and in vivo. We also found that Sp1 regulated miR-665 expression in endothelial cells. Our findings demonstrated that miR-665 played an important role in heart failure via damaging coronary microvessel angiogenesis, and suggested that miRNA-based ...Continue Reading

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Citations

Oct 20, 2019·Cell Cycle·Wen-Cai ZhangLuo-Sha Zhao
Jan 28, 2020·Circulation·Clarissa Pedrosa Costa GomesUNKNOWN EU-CardioRNA COST Action (CA17129)
Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Timo Z Nazari-ShaftiMaximilian Y Emmert
Mar 22, 2019·Frontiers in Cardiovascular Medicine·Rajinikanth GogirajuKatrin Schäfer
Dec 19, 2018·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·David StejskalMarek Svestak
Feb 9, 2021·Circulation Research·Jiahui FanDao Wen Wang
Aug 14, 2021·Frontiers in Cell and Developmental Biology·Tianbin ChenLi Zhang
Jun 2, 2020·Molecular Therapy. Methods & Clinical Development·Xiang NieChen Chen

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Methods Mentioned

BETA
transfection
co-immunoprecipitation
PCR
biopsy
Fluorescence
Assay
light microscopy

Software Mentioned

Image J
rnahybrid
SPSS

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