miR-665 promotes hepatocellular carcinoma cell migration, invasion, and proliferation by decreasing Hippo signaling through targeting PTPRB

Cell Death & Disease
Yuanchang HuXuehao Wang

Abstract

Growing evidence suggests that aberrant microRNA (miRNA) expression contributes to hepatocellular carcinoma (HCC) development and progression. However, the potential role and mechanism of miR-665 in the progression of liver cancer remains largely unknown. Our current study showed that miR-665 expression was upregulated in HCC cells and tissues. High expression of miR-665 exhibited more severe tumor size, vascular invasion and Edmondson grading in HCC patients. Gain- or loss-of-function assays demonstrated that miR-665 promoted cell proliferation, migration, invasion, and the epithelial-mesenchymal transition (EMT) of HCC cells in vitro and in vivo. Tyrosine phosphatase receptor type B (PTPRB) was downregulated in HCC tissues, and was negatively correlated with miR-665 expression. Through western blotting and luciferase reporter assay, PTPRB was identified as a direct downstream target of miR-665. Restoration of PTPRB reverses the effects of miR-665 on HCC migration, invasion, and cell proliferation. A mechanistic study showed that PTPTRB mediated the functional role of miR-665 through regulation of the Hippo signaling pathway. In conclusion, our results suggested that miR-665 was a negative regulator of the PTPRB and could prom...Continue Reading

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Citations

Oct 2, 2019·Molecular Genetics & Genomic Medicine·Xiwang YingYuanhui Zhang
Apr 11, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jianrong WuRanran Tang
Jul 26, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Li YuanShanming Ruan
Apr 8, 2020·Cells·Hyunwoo KimBuHyun Youn
Apr 10, 2019·Journal of Translational Medicine·Yanyan Han
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei ZhangChangjiang Liu
Dec 1, 2020·Cellular Signalling·Priyanka SamjiKarunagaran Devarajan
Jan 12, 2021·Developmental Biology·Frank SzulzewskyValeri Vasioukhin
Apr 4, 2021·Biomedicines·Na-Hyun LeeJeongeun Hyun
Apr 27, 2021·Cancer Biomarkers : Section a of Disease Markers·Hanyu ZhangHongyu Yang
Jul 3, 2021·Frontiers in Bioengineering and Biotechnology·Ogechi OgokeNatesh Parashurama

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Methods Mentioned

BETA
PCR
transfection
flow cytometry
xenograft
xenografts

Software Mentioned

Targetscan
miRanda

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