MiR-696 Regulates C2C12 Cell Proliferation and Differentiation by Targeting CNTFRα

International Journal of Biological Sciences
Han WangRuihua Huang

Abstract

Micro-696 (miR-696) has been previously known as an exercise related miRNA, which has a profound role in fatty acid oxidation and mitochondrial biogenesis of skeletal muscle. However, its role in skeletal myoblast proliferation and differentiation is still unclear. In this study, we found that miR-696 expressed highly in skeletal muscle and reduced during C2C12 myoblasts differentiation. MiR-696 overexpression repressed C2C12 myoblast proliferation and myofiber formation, while knockdown of endogenous miR-696 expression showed opposite results. During myogenesis, we observed an inversed expression pattern between miR-696 and CNTFRα invitro, and demonstrated that miR-696 could specifically target CNTFRα and repress the expression of CNTFRα. Additionally, we further found that knockdown of CNTFRα suppressed the proliferation and differentiation of C2C12 cells. Taking all things together, we propose a novel insight that miR-696 down-regulates C2C12 cell myogenesis by inhibiting CNTFRα expression.

Citations

Apr 17, 2019·Journal of Applied Physiology·Anthony J SannicandroKatarzyna Goljanek-Whysall
Jan 15, 2020·Development·Mateusz DobrowolskiShanthini Sockanathan
Jun 24, 2019·Journal of Cellular Physiology·Meng XuZhiqing Huang
Dec 4, 2020·Neural Regeneration Research·Shi-Jia YuJuan Feng
Jan 6, 2021·Journal of Cellular Biochemistry·Yan-Hua DongMing Cang

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Methods Mentioned

BETA
transfection
flow cytometry

Software Mentioned

miRanda
ModFit
SPSS
TargetScan
ImageJ
PicTar

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