MiR-7 reduces the BCSC subset by inhibiting XIST to modulate the miR-92b/Slug/ESA axis and inhibit tumor growth

Breast Cancer Research : BCR
Miao LiJun Dou

Abstract

Breast cancer stem cells (BCSCs) are typically seed cells of breast tumor that initiate and maintain tumor growth. MiR-7, as a cancer inhibitor, decreases the BCSC subset and inhibits tumor progression through mechanisms that remain unknown. We examined miR-7 expression in breast cancer and developed a BCSC-driven xenograft mouse model, to evaluate the effects of miR-7 overexpression on the decrease of the BCSC subset in vitro and in vivo. In addition, we determined how miR-7 decreased the BCSC subset by using the ALDEFLUOR, lentivirus infection, dual-luciferase reporter, and chromatin immunoprecipitation-PCR assays. MiR-7 was expressed at low levels in breast cancer tissues compared with normal tissues, and overexpression of miR-7 directly inhibited lncRNA XIST, which mediates the transcriptional silencing of genes on the X chromosome, and reduced epithelium-specific antigen (ESA) expression by increasing miR-92b and inhibiting slug. Moreover, miR-7 suppressed CD44 and ESA by directly inhibiting the NF-κB subunit RELA and slug in breast cancer cell lines and in BCSC-driven xenografts, which confirmed the antitumor activity in mice injected with miR-7 agomir or stably infected with lenti-miR-7. The findings from this study unco...Continue Reading

References

Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Oct 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jiangbing ZhouYing Zhang
Sep 8, 2012·Genome Research·Jennifer HarrowTim J Hubbard
Feb 7, 2013·Cancer Research·Hiroshi OkudaKounosuke Watabe
Sep 11, 2013·RNA Biology·Markus Kretz
Nov 22, 2013·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Dengyu ChenJun Dou
Jul 23, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Felipe AdesMartine Piccart
Sep 8, 2016·Nature·Deepak P PatilSamie R Jaffrey
Oct 14, 2017·Cancer Genetics·Weiliang SunJunming Guo
Jun 21, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chaofeng MuHaifa Shen
Jun 23, 2018·Photodiagnosis and Photodynamic Therapy·Alireza GhaderMohammad Hosein Majles Ara
Jun 27, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Alison M NagleAdrian V Lee

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Citations

Jan 10, 2021·Pharmacology & Therapeutics·Juntao LiErxi Wu
Feb 19, 2021·Regenerative Therapy·Kosuke YoshidaTakahiro Ochiya
Apr 4, 2021·Biomedicines·Petra KoraćMaja Matulić
Apr 14, 2021·Cancer Letters·Jaganathan VenkateshPaola Marcato
Apr 17, 2021·Essays in Biochemistry·Huiwen Yan, Pengcheng Bu
Apr 21, 2021·Experimental and Molecular Pathology·Meng-Qi WangPeng Gao
Apr 29, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Deng NingLi Jiang
Aug 13, 2021·World Journal of Surgical Oncology·Yu DuLusheng Guo
Oct 7, 2021·Bioscience Reports·Ying Jing, Donghai Li

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Methods Mentioned

BETA
xenografts
PCR
Assay
immunoprecipitation
ChIP
pulldown
transfection
flow cytometry
antisense
pull down

Software Mentioned

TargetScan
miRcode

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