Abstract
This study aimed to clarify the regulation role of miR-708 and miR-335-3p in retinal ganglion cell (RGC) autophagy and apoptosis in glaucoma. Chronic glaucoma mice were established by laser photocoagulation. RGCs were isolated and transfected with a series of plasmids and the cultured in 60 mmHg pressure. miR-335-3p, miR-708, and ATG3 mRNA expressions were detected by qRT-PCR. Protein levels of ATG3, autophagy-related protein LC3, and p62 were detected by Western blot. The apoptosis of RGCs was detected by flow cytometry. The regulation role of miR-335-3p/miR-708 in ATG3 was confirmed by the dual-luciferase reporter gene. The expressions of several miRNAs were measured in retinal tissues from chronic glaucoma mice and RGCs under pressure conditions, and results showed that both miR-335-3p and miR-708 were down-regulated. Besides, the inhibition of miR-708 and miR-335-3p induced the apoptosis of RGCs through promoting autophagy. Also, miR-708 and miR-335-3p could bind to ATG3 and targeted regulated ATG3. Furthermore, the interference with miR-708/miR-335-3p induced RGC apoptosis by up-regulating ATG3 to promote autophagy. In general, the down-regulation of miR-708 and miR-335-3p contributed to the apoptosis of RGCs through promo...Continue Reading
References
Jan 27, 2009·Cell·David P Bartel
Aug 2, 2011·Annual Review of Cell and Developmental Biology·Noboru MizushimaYoshinori Ohsumi
Apr 6, 2012·Cell Death & Disease·H Y Lopilly ParkC K Park
Jan 17, 2013·Investigative Ophthalmology & Visual Science·Liang FengXiaorong Liu
Sep 26, 2013·Nature Reviews. Immunology·Vojo DereticShizuo Akira
Oct 15, 2013·Acta Physiologica·D BloembergJ Quadrilatero
Dec 10, 2013·The Journal of Comparative Neurology·Allen R RodriguezNicholas C Brecha
Apr 1, 2014·Experimental Eye Research·Xinyu XuYingbin Ge
Nov 20, 2014·BMC Molecular Biology·Ning KongBin Li
Mar 11, 2015·Clinical & Experimental Ophthalmology·Dana MorzaevNitza Goldenberg-Cohen
Jun 11, 2015·Autophagy·Byong Hoon YooKirill V Rosen
Feb 26, 2016·FEBS Letters·Wen LiDu Feng
Sep 23, 2016·Nature Reviews. Disease Primers·Robert N WeinrebKeith R Martin
Nov 22, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Naghmeh ShirafkanBehzad Baradaran
Feb 9, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rui LiHongping Cui
May 2, 2018·EMBO Reports·Michal LubasLisa B Frankel
Aug 23, 2018·Biochemical and Biophysical Research Communications·Jingwen YangXiaoqin Luo
Sep 6, 2018·American Journal of Physiology. Cell Physiology·Xin-Gang NieFeng Gao
Sep 8, 2018·Scientific Reports·Hae-Young Lopilly ParkChan Kee Park
Nov 16, 2018·Cellular Signalling·Hui PengE Song
Dec 12, 2018·Cancer Cell International·Tao WangQuan Zhang
Jan 8, 2019·Experimental Eye Research·Johanna M WalzAndreas Stahl
May 24, 2019·Cancer Management and Research·Fei WangZhenyu Yin
Jun 28, 2019·Experimental Neurology·Huadong FanShao-Yu Chen
Oct 2, 2019·Journal of Neuroinflammation·Anitha KrishnanMeredith Gregory-Ksander