MiR-874 alleviates renal injury and inflammatory response in diabetic nephropathy through targeting toll-like receptor-4

Journal of Cellular Physiology
Tao YaoXiaoyan Wu

Abstract

Diabetic nephropathy (DN) is a kind of diabetic complication with capillary damage, and its pathogenesis remains obscure. Recently, microRNAs have been identified as diagnostic biomarkers in various diseases including DN. Toll-like receptor 4 (TLR4) contributes to inflammation, and it has been implicated in diabetes pathophysiology. This study was designed to investigate the role of miR-874 and TLR4 in a streptozotocin (STZ)-induced DN rat model and glucose-induced mouse podocyte model. In the current study, we reported that miR-874 was markedly downregulated in DN rats and glucose-induced mouse podocytes compared with the corresponding control groups with the activation of TLR4. In addition, we observed that overexpression of miR-874 was able to alleviate renal injury in DN rats. The cell counting kit (CCK-8) assay and 5-Ethynyl-2'-deoxyuridine (EdU) assay demonstrated that glucose simulation significantly inhibited podocyte proliferation and induced cell apoptosis, which can be reversed by miR-874 mimics significantly. Notably, miR-874 overexpression dramatically attenuated the inflammatory response, indicated by the decreased levels of interleukin-6, L-1β, and tumor necrosis factor α (TNF-α). Finally, the binding correlation...Continue Reading

References

Jan 1, 1993·Annual Review of Medicine·S AdlerA Artishevsky
Nov 28, 2002·JAMA : the Journal of the American Medical Association·Matthew J Sheetz, George L King
Apr 23, 2003·Journal of the American Society of Nephrology : JASN·Thoralf WendtAnn Marie Schmidt
Oct 13, 2006·Journal of the American Society of Nephrology : JASN·Mauro AbbateGiuseppe Remuzzi
Nov 23, 2006·Current Diabetes Reports·Carmen Mora, Juan F Navarro
Sep 18, 2010·Journal of the American Society of Nephrology : JASN·Huiling WuSteven J Chadban
May 4, 2011·Nature Reviews. Nephrology·Juan F Navarro-GonzálezJavier García-Pérez
Oct 25, 2011·Journal of the American Society of Nephrology : JASN·Miao LinSydney C W Tang
Aug 10, 2012·American Journal of Physiology. Renal Physiology·Harmeet KaurIshwarlal Jialal
Apr 16, 2013·Biochemical and Biophysical Research Communications·Divya KesanakurtiChandramu Chetty
Jun 26, 2013·Asian Pacific Journal of Tropical Medicine·Si-Yang LiuJing Chen
Sep 19, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wen-gang ShaGuo-Yuan Lu
Dec 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dong DongGuishan Gu
Mar 15, 2016·Current Diabetes Reports·Kate SimpsonTimothy Bowen
Feb 16, 2018·Respiratory Physiology & Neurobiology·Meihua SunLianhua Jin

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Citations

Sep 7, 2019·BMC Complementary and Alternative Medicine·Yan ChenWeisheng Feng
May 1, 2020·Clinical and Translational Gastroenterology·Susrutha Puthanmadhom NarayananAdil E Bharucha
Sep 11, 2020·International Journal of Molecular Sciences·Linsey J F PetersEmiel P C van der Vorst
Mar 21, 2020·Frontiers in Pharmacology·Tamil Selvi LoganathanNoraidatulakma Abdullah
Feb 13, 2021·Frontiers in Pharmacology·Yue-Yu GuHui-Yao Lan
Nov 17, 2020·Frontiers in Genetics·Hiroki IshiiYoshiyuki Morishita
May 25, 2021·Frontiers in Physiology·Bao-Yi ShaoHai-Yong Chen

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