miR-9 Mediates CALHM1-Activated ATP-P2X7R Signal in Painful Diabetic Neuropathy Rats

Molecular Neurobiology
Wenjie LiuXiaoling Hu

Abstract

In this study, we planned to illuminate the mechanisms of the expression and function of CALHM1 in painful diabetic neuropathy (PDN). PDN rat model was constructed. The expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons was detected. The correlation between the level of CALHM1 mRNA and 50 % PWT and the relationship between the expression of CALHM1 and miR-9 in rat spinal dorsal horn neurons were statistically analyzed. The effect of miR-9 and CALHM1 on each other's expression in PDN rat spinal dorsal horn neurons were tested by qRT-PCR or Western blot. The co-culture system of neurons and glias from PDN rat spinal dorsal horn was constructed. The concentration of calcium and ATP as well as the expression of P2X7 receptor regulated by CALHM1 and miR-9 in PDN rat spinal dorsal horn neurons was measured. The results showed that the expression of CALHM1 was increased in PDN rat compared with controls, while its mRNA level was negatively correlated with 50 % PWT. miR-9, which was also upregulated in the spinal dorsal horn neurons of PDN rats, was positively correlated with the expression of CALHM1. The concentration of calcium and ATP as well as the expression of P2X7 receptor in glias was also increased in PDN rats. T...Continue Reading

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Citations

Nov 23, 2017·Purinergic Signalling·Geoffrey Burnstock, Gillian E Knight
Mar 14, 2020·Cell & Bioscience·Danwen WangZhiling Sun
Jan 1, 2019·World Journal of Experimental Medicine·Eleni XourgiaAndreas Melidonis
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Aug 2, 2018·Frontiers in Cellular Neuroscience·Liangcai ZhaoHongchang Gao
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Sep 22, 2019·International Journal of Molecular Sciences·Raffaele Simeoli, Alessandra Fierabracci
Nov 17, 2020·Frontiers in Neurology·Baoyan FanXian Shuang Liu
Jun 3, 2021·International Journal of Molecular Sciences·Milena JankovicDragana Bogicevic

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Methods Mentioned

BETA
Assay
electrophoresis
transfection

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TargetScan

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