MiR-9 promotes angiogenesis of endothelial progenitor cell to facilitate thrombi recanalization via targeting TRPM7 through PI3K/Akt/autophagy pathway.

Journal of Cellular and Molecular Medicine
Dong-Ming ZhouWen-Dong Li

Abstract

Endothelial progenitor cells (EPCs) have emerged as a promising therapeutic choice for thrombi recanalization. However, this role of EPCs is confined by some detrimental factors. The aim of this study was to explore the role of the miR-9-5p in regulation of the proliferation, migration and angiogenesis of EPCs and the subsequent therapeutic role in thrombosis event. Wound healing, transwell assay, tube formation assay and in vivo angiogenesis assay were carried out to measure cell migration, invasion and angiogenic abilities, respectively. Western blot was performed to elucidate the relationship between miR-9-5p and TRPM7 in the autophagy pathway. It was found that miR-9-5p could promote migration, invasion and angiogenesis of EPCs by attenuating TRPM7 expression via activating PI3K/Akt/autophagy pathway. In conclusion, miR-9-5p, targets TRPM7 via the PI3K/Ak/autophagy pathway, thereby mediating cell proliferation, migration and angiogenesis in EPCs. Acting as a potential therapeutic target, miR-9-5p may play an important role in the prognosis of DVT.

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Citations

Aug 28, 2020·Frontiers in Cell and Developmental Biology·Xinghong YaoYe Zeng
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Nov 5, 2020·Burns : Journal of the International Society for Burn Injuries·Yan ZhiJia Zhang

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Methods Mentioned

BETA
confocal
flow cytometry
transfection
PCR
electrophoresis
confocal microscopy

Software Mentioned

SPSS
TargetScan

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