MiR-9 promotes tumorigenesis and angiogenesis and is activated by MYC and OCT4 in human glioma

Journal of Experimental & Clinical Cancer Research : CR
Xu ChenTao Wang

Abstract

Glioma, characterized by its undesirable prognosis and poor survival rate, is a serious threat to human health and lives. MicroRNA-9 (miR-9) is implicated in the regulation of multiple tumors, while the mechanisms underlying its aberrant expression and functional alterations in human glioma are still controversial. Expressions of miR-9 were measured in GEO database, patient specimens and glioma cell lines. Gain- and loss-of-function assays were applied to identify the effects of miR-9 on glioma cells and HUVECs in vitro and in vivo. Potential targets of miR-9 were predicted by bioinformatics and further verified via in vitro experiments. Transcriptional regulation of miR-9 by MYC and OCT4 was determined in glioma cells. MiR-9 was frequently up-regulated in glioma specimens and cells, and could significantly enhance proliferation, migration and invasion of glioma cells. In addition, miR-9 could be secreted from glioma cells via exosomes and was then absorbed by vascular endothelial cells, leading to an increase in angiogenesis. COL18A1, THBS2, PTCH1 and PHD3 were verified as the direct targets of miR-9, which could elucidate the miR-9-induced malignant phenotypes in glioma cells. MYC and OCT4 were able to bind to the promoter re...Continue Reading

References

Apr 3, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shaopeng YuanYongzhang Luo
Aug 2, 2008·The New England Journal of Medicine·Patrick Y Wen, Santosh Kesari
Nov 6, 2008·Journal of Experimental & Clinical Cancer Research : CR·David M NeskeyPaula J McKeown-Longo
Mar 21, 2009·Journal of Cellular and Molecular Medicine·Tri Minh Bui NguyenS Ramakrishnan
Oct 2, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Xiangyu MaXingang Li
Aug 23, 2011·The EMBO Journal·Daniel SchraivogelGunter Meister
May 4, 2012·Cold Spring Harbor Perspectives in Medicine·Patrick R Lawler, Jack Lawler
Jan 10, 2014·Journal of Cellular Physiology·Er-Bao BianBing Zhao
Aug 19, 2014·Oncogene·H I SuzukiK Miyazono
Dec 4, 2014·Cancer Treatment and Research·Quinn T OstromJill S Barnholtz-Sloan
Aug 1, 2015·Brain Pathology·Marietta WolterGuido Reifenberger
Sep 8, 2015·Protein and Peptide Letters·Kyu-Yeon HanDimitri T Azar
Sep 12, 2015·American Journal of Physiology. Cell Physiology·Sanjukta ChakrabortyMariappan Muthuchamy
Mar 12, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yudan Chi, Dongming Zhou
May 26, 2016·Stem Cells and Development·María Concepción Cruz-SantosAlfredo Cruz-Ramírez
May 27, 2016·International Journal of Medical Sciences·Yan MouXin Sun
Jun 2, 2016·Cancer Research·Jianfei XueSuyun Huang
Jun 28, 2016·QJM : Monthly Journal of the Association of Physicians·S AzizmohammadiM Seifoleslami
Oct 8, 2016·World Journal of Surgical Oncology·Chenghua DuChang Liu
Oct 11, 2016·Journal of Experimental Neuroscience·Balachandar Radhakrishnan, A Alwin Prem Anand
Oct 26, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Ritva HeljasvaaraTaina Pihlajaniemi
Jan 28, 2017·Cell Proliferation·Hong-Yu GaoDong-Sheng Pei
Apr 22, 2017·Journal of Neuropathology and Experimental Neurology·Heather AmesFausto J Rodriguez
Aug 16, 2017·Experimental and Therapeutic Medicine·Pingyuan BuDan Xu
Aug 17, 2017·Cell Reports·Romain MadelainePhilippe Mourrain

❮ Previous
Next ❯

Citations

Aug 5, 2020·Cell Communication and Signaling : CCS·Amir B GhaemmaghamiMichael R Hamblin
Sep 25, 2019·International Journal of Molecular Sciences·Francesco IngenitoGerolama Condorelli
Jan 11, 2020·Frontiers in Oncology·Abraham Pedroza-TorresLuis A Herrera
Apr 18, 2020·Journal of Experimental & Clinical Cancer Research : CR·Shiming TanQianjin Liao
Sep 23, 2020·Journal of Experimental & Clinical Cancer Research : CR·Shuangshuang MaoJie He
Mar 31, 2020·Journal of Hematology & Oncology·Ming YiKongming Wu
Oct 22, 2020·International Journal of Molecular Sciences·Erna Marija MeškytėYari Ciribilli
Dec 4, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Bikash Chandra Jena, Mahitosh Mandal
Dec 18, 2020·Frontiers in Oncology·Tiziana AnneseDomenico Ribatti
Aug 14, 2020·Vascular Pharmacology·Soudeh Ghafouri-FardMohammad Taheri
Apr 20, 2021·Frontiers in Immunology·Yunkai Yang, Yan Wang
Feb 8, 2020·Journal of the Neurological Sciences·Shiming LiuHaowen Wang
Jun 17, 2021·Molecular Medicine Reports·Qi Zheng, Wei Hou
Aug 8, 2021·International Journal of Molecular Sciences·Ximena VásquezVerónica Palma
Sep 15, 2021·Technology in Cancer Research & Treatment·Cheng-You JiaDa Fu
Aug 7, 2019·Seminars in Cancer Biology·Francesca OrsoDaniela Taverna

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE15824
GSE93850

Methods Mentioned

BETA
transfection
ELISA
PCR
light microscopy
xenograft
immunoprecipitation
Assay
flow cytometry
force
ChIP

Software Mentioned

Targetscan
SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.