miR-93-5p enhance lacrimal gland adenoid cystic carcinoma cell tumorigenesis by targeting BRMS1L

Cancer Cell International
Jie HaoHong Zhang

Abstract

Lacrimal adenoid cystic carcinoma (LACC) is one of the most common malignancies that affects lacrimal gland. MicroRNAs are known to play a crucial role as oncogenes or tumor suppressors. Specifically, miR-93 has been reported to play a crucial role in colorectal, breast, pancreatic, lung cancer and hepatocellular carcinoma. However, the role of miR-93 in LACC and the potential molecular mechanisms involved remain unknown. Therefore, we took the challenge to determine the involvement of miR-93 in the LACC by targeting BRMS1L. A total of 5 adenoid cystic carcinoma (ACC) of lacrimal gland patient tissues and their plasma were examined. Three normal lacrimal glands and three normal serums were collected as a control group. After surgical resection, the specimens were preserved in liquid nitrogen and stored at - 80 °C until RNA extraction. Afterwards, LACC cells with miR-93-5p overexpression were subjected to qRT-PCR and western blot for epithelial-mesenchymal transition (EMT) markers levels. Ability of LACC cell migration, invasion, proliferation and apoptosis was examined by wounded healing, transwell, CCK-8 and apoptosis assays. Afterwards, TargetScan was used to predict putative targets of miR-93-5p. Then, the examination was pe...Continue Reading

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Citations

Mar 22, 2019·Bioinformatics·Malik YousefJens Allmer
May 24, 2019·World Journal of Clinical Cases·Lin-Lin SuXiao-Ying Xue
Jul 3, 2020·Cancer Cell International·Milad AshrafizadehSaeed Samarghandian

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Methods Mentioned

BETA
xenograft
PCR
transfections
flow cytometry
Assay
transfection
electrophoresis
xenografts

Software Mentioned

TargetScan
Image
Studio

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