MiR-93 Targeting EphA4 Promotes Neurite Outgrowth from Spinal Cord Neurons

Journal of Molecular Neuroscience : MN
Xiaogang ChenXiaoqing Lu

Abstract

The failure of neurite outgrowth in the adult mammalian spinal cord injury is thought to be attributed to the intrinsic growth ability of mature neurons. Ephrin/Eph system is a major growth regulator of many axonal guidance processes. EphA4 is expressed specifically in traumatic central nervous system (CNS) and dynamically regulate target gene expression, suggesting that it may be associated with neural regeneration. Here, we found an alteration in temporal expression of miR-93 following a contusive spinal cord injury (SCI) in adult rats. The messenger RNA (mRNA) expression level of miR-93 was upregulated and the protein expression levels of EphA4, p-Ephexin, and active RhoA were all decreased in traumatic spinal cord relative to those with an intact spinal cord. Infection of cultured spinal cord neurons (SCNs) with miR-93 mimic led to neuronal growth promotion and decreased levels of EphA4, p-Ephexin, and active RhoA protein expression. Dual-luciferase reporter assay confirmed that miR-93 bound to the three prime untranslated region (3' UTR) of EphA4 and inhibited the expression of EphA4 mRNA. These findings provide evidence that miR-93 inhibits EphA4 expression, decreased EphA4 expression could promote neurite outgrowth in SC...Continue Reading

References

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·M DottoriA W Boyd
Feb 17, 2001·Current Opinion in Neurobiology·B J Dickson
Aug 21, 2002·Trends in Cell Biology·Chad A Cowan, Mark Henkemeyer
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Juha-Pekka Himanen, Dimitar B Nikolov
Apr 1, 2003·Annals of Neurology·Simone Di GiovanniAlan I Faden
Jun 12, 2003·Cell Transplantation·Christopher A WillsonScott R Whittemore
Oct 3, 2003·Trends in Neurosciences·Florence M Bareyre, Martin E Schwab
Sep 15, 2004·Current Opinion in Cell Biology·Rüdiger Klein
Nov 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yona GoldshmitAnn M Turnley
Apr 26, 2005·Neuron·Mustafa SahinMichael E Greenberg
Aug 23, 2005·Brain Research. Brain Research Reviews·Albert Martínez, Eduardo Soriano
Jun 16, 2006·Brain Research Reviews·Yona GoldshmitAnn Turnley
Oct 5, 2006·Molecular Cell·Julide BilenNancy M Bonini
Sep 27, 2007·Molecular Cancer·William C S Cho
Jan 31, 2008·RNA·Mads BakSakari Kauppinen
Feb 19, 2008·Genes & Development·Dina Arvanitis, Alice Davy
Oct 31, 2008·Current Protocols in Neuroscience·Simon W Moore, Timothy E Kennedy
Jul 7, 2009·Experimental Neurology·Nai-Kui LiuXiao-Ming Xu
Sep 5, 2009·Neuromolecular Medicine·Joachim SilberJ Graeme Hodgson

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Citations

Nov 8, 2017·Oxidative Medicine and Cellular Longevity·Wen LiDu Feng
Feb 20, 2018·Experimental and Therapeutic Medicine·Jin-Shan YangGang Wu
Jul 3, 2020·Cancer Cell International·Milad AshrafizadehSaeed Samarghandian
Jul 8, 2020·Pharmaceuticals·Sarah BaudetXavier Nicol
Aug 8, 2021·International Journal of Molecular Sciences·Miguel E Domínguez-Romero, Paula G Slater

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