miR-99a reveals two novel oncogenic proteins E2F2 and EMR2 and represses stemness in lung cancer

Cell Death & Disease
Andrea FelicianoMatilde E LLeonart

Abstract

Lung cancer is one of the most aggressive tumours with very low life expectancy. Altered microRNA expression is found in human tumours because it is involved in tumour growth, progression and metastasis. In this study, we analysed microRNA expression in 47 lung cancer biopsies. Among the most downregulated microRNAs we focussed on the miR-99a characterisation. In vitro experiments showed that miR-99a expression decreases the proliferation of H1650, H1975 and H1299 lung cancer cells causing cell cycle arrest and apoptosis. We identified two novel proteins, E2F2 (E2F transcription factor 2) and EMR2 (EGF-like module-containing, mucin-like, hormone receptor-like 2), downregulated by miR-99a by its direct binding to their 3'-UTR. Moreover, miR-99a expression prevented cancer cell epithelial-to-mesenchymal transition (EMT) and repressed the tumourigenic potential of the cancer stem cell (CSC) population in both these cell lines and mice tumours originated from H1975 cells. The expression of E2F2 and EMR2 at protein level was studied in 119 lung cancer biopsies. E2F2 and EMR2 are preferentially expressed in adenocarcinomas subtypes versus other tumour types (squamous and others). Interestingly, the expression of E2F2 correlates with ...Continue Reading

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Citations

Oct 18, 2018·PloS One·Anselm A DerdaThomas Thum
Feb 6, 2019·Cancers·Randall J Ruch
Oct 31, 2018·Molecular & Cellular Proteomics : MCP·Etna AbadMatilde E Lleonart
May 20, 2018·Scientific Reports·Mariana S ParahulevaGerhild Euler
May 6, 2019·Carcinogenesis·Yoelsis Garcia-MayeaMatilde E LLeonart
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Nov 27, 2019·Journal of the Chinese Medical Association : JCMA·Afeez Adekunle IsholaMong-Lien Wang
Sep 27, 2019·Experimental and Therapeutic Medicine·Bingjun HeGuangmao Yu
Oct 23, 2019·Journal of Cellular Physiology·Tao ChenLei Guo
Nov 24, 2020·Frontiers in Oncology·Andrea FelicianoMatilde E LLeonart
Nov 25, 2020·Wiley Interdisciplinary Reviews. RNA·Joseph Eniafe, Shuai Jiang
Jan 29, 2021·Cancer Communications·Guangtao PanShenglan Yang
Feb 1, 2019·Molecular & Cellular Proteomics : MCP·Etna AbadMatilde E Lleonart
Nov 11, 2020·Cancers·Yoelsis Garcia-MayeaMatilde E LLeonart
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May 29, 2021·MicroRNA·Younes El FouniniFadila Guessous

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Methods Mentioned

BETA
transfection
FACS
biopsies
Feature Extraction
PCR
protein
electrophoresis
fluorescence microscopy
fluorescence-activated cell sorting

Software Mentioned

limma
SPSS
FACS Express
Feature Extraction

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