miR-sc3, a Novel MicroRNA, Promotes Schwann Cell Proliferation and Migration by Targeting Astn1

Cell Transplantation
Sheng YiShiying Li

Abstract

MicroRNAs (miRNAs, miRs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level. We have previously identified a group of novel miRNAs in proximal sciatic nerve after sciatic nerve transection by Solexa sequencing, and miR-sc3 is a member of the group. In this study, we aimed to investigate the effects of miR-sc3 on phenotypic modulation of Schwann cells (SCs). miR-sc3 was highly expressed in the injured nerve after sciatic nerve transection. An increased and decreased expression of miR-sc3 promoted and reduced the proliferation and migration of primary SCs, respectively. miR-sc3 directly targeted astrotactin 1 (Astn1) and led to translational suppression of Astn1. There was an inverse association between the time-dependent expressions of miR-sc3 and Astn1 in proximal sciatic nerve after sciatic nerve transection. Overall, miR-sc3 affected SC proliferation and migration by targeting Astn1, thus playing the regulatory role in peripheral nerve regeneration.

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Citations

Nov 25, 2017·Neural Regeneration Research·Sheng YiSong-Lin Zhou
Aug 6, 2018·Neurochemical Research·Pan WangTianmei Qian
Feb 2, 2018·Molecular and Cellular Biochemistry·Tianmei QianSheng Yi
Sep 19, 2018·Neurorehabilitation and Neural Repair·Liming QingXiaofeng Jia
Jan 12, 2019·The European Journal of Neuroscience·Guozhen LuXianghui Zhao
Apr 17, 2019·Experimental and Therapeutic Medicine·Chu ChenTianmei Qian
Nov 15, 2018·Frontiers in Physiology·Xinghui WangSheng Yi
Apr 5, 2020·Neural Regeneration Research·Xiao-Kun GuHui Xu
May 16, 2019·Neural Regeneration Research·Xi-Meng JiTian-Mei Qian
Jun 2, 2018·BioMed Research International·Eun Jung Sohn, Hwan Tae Park
Nov 17, 2020·Frontiers in Neurology·Baoyan FanXian Shuang Liu
Mar 12, 2021·Developmental Biology·Bridget T Jacques-FrickeLaura S Gammill

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Methods Mentioned

BETA
Transfection
PCR
Protein Assay
transfect

Software Mentioned

Grab
Gelwork
SPSS
it
Solexa

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