miR‑1 inhibits the progression of colon cancer by regulating the expression of vascular endothelial growth factor

Oncology Reports
Dehua ZhuJianping Zhou

Abstract

MicroRNA (miR)‑1 is associated with various human malignancies through repressing tumor growth, migration and angiogenesis. Recently, high‑throughput transcriptional profiling confirmed that miR‑1 is markedly downregulated in metastatic colorectal cancer; however, its biological functions and the specific underlying mechanisms in colorectal cancer (CRC) require further investigation. In this study, the expression of miR‑1 in 111 CRC and paired normal tissue samples was measured using quantitative polymerase chain reaction analysis, and the association between miR‑1 expression and clinical characteristics was evaluated. miR‑1 was found to be significantly downregulated in CRC tissues compared with paired normal tissues, and in CRC cell lines compared with non‑cancer cells (P<0.001), and was negatively associated with tumor size (P=0.001), differentiation (P=0.011), lymph node metastasis (P=0.001) and TNM stage (P=0.001). Further experiments revealed that miR‑1 inhibited the migration and invasion of HCT116 and ClonA1 cells, and inhibited cell proliferation by affecting the cell cycle. Vascular endothelial growth factor (VEGF) was found to be a potential target of miR‑1 by biological prediction, and further investigation confirme...Continue Reading

Citations

Nov 7, 2019·International Journal of Molecular Sciences·Huawei LiuXiaoping Pan
Jul 3, 2020·International Journal of Molecular Sciences·Paulína PidíkovaIveta Herichova
Apr 4, 2021·Biomedicines·Ying-Chin LinJung-Chun Lin
Aug 26, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Aya Aly AshrafHeba Mohamed Shawky

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Methods Mentioned

BETA
transfection
ELISA
electrophoresis
FACS
flow cytometry

Software Mentioned

Miranda
SPSS
PicTar
TargetScan

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