miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings

Scientific Reports
Xiao Lin JiaXin Zhong Zhang

Abstract

In higher plants, miR156 regulates the vegetative phase change via the target SBP/SPL genes. The regulation of miR156 during ontogenetic processes is not fully understood. In the apple genome, of 31 putative MdMIR156 genes that encode pre-miR156, seven were dominantly expressed. However, the transcript levels of only MdMIR156a5 and MdMIR156a12 decreased significantly during the vegetative phase change, which was consistent with the mature miR156 level, indicating that miR156 is under transcriptional regulation. Leaf H2O2 content was higher in the adult phase than in the juvenile phase because of excess H2O2 accumulation in chloroplasts. When in vitro shoots were treated with menadione, diphenyleneiodonium, L-2-oxothiazolidine-4-carboxylic acid or buthionine sulphoximine, the expressions of MdMIR156a5, MdMIR156a12, and as well miR156 were coordinated with reduced glutathione (GSH) contents and glutathione/glutathione disulfide ratio but not H2O2 contents. Alteration of miR156 expression level by MdMIR156a6-overexpressing or miR156-mimetic transgenic Nicotiana benthamiana did not cause a corresponding change in reactive oxygen species or GSH status. Collectively, the results indicate that the vegetative phase change in apple is c...Continue Reading

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Citations

Jul 30, 2020·The Plant Journal : for Cell and Molecular Biology·Leonie Verhage
Aug 12, 2020·Plant Cell Reports·Zijie ZhangYun Li
Sep 21, 2018·Frontiers in Plant Science·Anna M WójcikMałgorzata D Gaj
Oct 5, 2018·Frontiers in Plant Science·Manuel Buendía-Monreal, C Stewart Gillmor
Jun 20, 2019·Frontiers in Plant Science·Muhammad Umair AhsanNeena Mitter
Jan 13, 2019·Plants·Angelo De PaolisSofia Caretto

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Methods Mentioned

BETA
transgenic
transmission electron microscopy
three hybrid

Software Mentioned

Primer
BLASTP
Primer Express
BLAST

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