MiR17 improves insulin sensitivity through inhibiting expression of ASK1 and anti-inflammation of macrophages

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Chen ZhangZhe Sun

Abstract

MicroRNAs (miRNAs) are involved in the pathological progression of various disease including type 2 diabetes (T2D). Chronic inflammation in adipose tissue is a cause of insulin resistance and T2D. MiR-17 palys an anti-inflammatory role in many biological processes. We hypothesized that miR-17 suppressed inflammatory macrophage that is related to insulin resistance in patients with T2D. Macrophage migration and secretion of inflammatory cytokines including TNF-α, IL-6 and IL-1β were detected through transwell migration assay and enzyme-linked immunosorbent assay, respectively. Insulin-stimulated glucose uptake was tested by the radioactivity of tritium-labeled glucose in 3T3-L1 adipocytes. Dual luciferase reporter gene assay was employed to evaluate the interaction between miR-17 and 3'UTR of ASK1. Our results showed that miR-17 inhibited macrophage infiltration and secretion of TNF-α, IL-6 and IL-1β. Moreover, insulin-stimulated glucose uptake of 3T3-L1 was suppressed by treatment with LPS-induced macrophage conditioned media (CM), whereas the opposite effect was showed after treatment with the CM of macrophages transfected with miR-17. Furthermore, we found that miR-17 directly prevented expression of ASK1 by binding to its 3'...Continue Reading

References

May 3, 2001·The New England Journal of Medicine·J TuomilehtoUNKNOWN Finnish Diabetes Prevention Study Group
Jan 30, 2004·EMBO Reports·Kohsuke TakedaHidenori Ichijo
Oct 8, 2004·The British Journal of Nutrition·Paul Trayhurn, I Stuart Wood
Nov 9, 2004·Trends in Immunology·Alberto MantovaniMassimo Locati
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Feb 14, 2006·The American Journal of Clinical Nutrition·Andrew S Greenberg, Martin S Obin
Mar 7, 2006·Microbes and Infection·Teruyuki HayakawaHidenori Ichijo
Jan 4, 2007·The Journal of Clinical Investigation·Carey N LumengAlan R Saltiel
Jan 18, 2007·The Journal of Biological Chemistry·Karim Bouzakri, Juleen R Zierath
Mar 29, 2007·The Journal of Cell Biology·Nao OsakaHidenori Ichijo
Feb 12, 2008·Journal of Cellular Physiology·Ping WangJaap Keijer
Aug 20, 2009·BMC Medical Genomics·Blanca M HerreraCecilia M Lindgren
Dec 4, 2009·International Reviews of Immunology·Enikö Sonkoly, Andor Pivarcsi
Jan 9, 2010·Biochimica Et Biophysica Acta·Sam Virtue, Antonio Vidal-Puig
Jan 26, 2010·Nature Reviews. Immunology·Ryan M O'ConnellDavid Baltimore
Jul 30, 2010·Molecular and Cellular Endocrinology·Xiao-Hui ChenXi-Rong Guo
Jan 21, 2011·Molecular and Cellular Biochemistry·M BalasubramanyamV Mohan
Mar 9, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miaomiao ZhangRongcun Yang
Jun 3, 2011·The Journal of Clinical Investigation·Kai SunPhilipp E Scherer
Oct 20, 2012·Nature Reviews. Drug Discovery·Eva van Rooij, Eric N Olson
Feb 5, 2013·Advances in Experimental Medicine and Biology·William RitchieStéphane Flamant
Mar 22, 2013·Frontiers in Immunology·Lucas PhilippePhilippe Georgel
Apr 26, 2013·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·N E Corral-FernándezD P Portales-Pérez
Oct 14, 2014·Frontiers in Immunology·Michael James KraakmanHélène L Kammoun
Sep 30, 2015·Archives of Biochemistry and Biophysics·Eugenio J AbenteS Hani Najafi-Shoushtari
Dec 15, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Daoyin DongPeixin Yang
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Marcelina SperlingKrystyna Czyzewska
Aug 19, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nahid AkhtarSalahuddin Ahmed
Nov 24, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C GuoX Gu

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Citations

May 16, 2019·Experimental and Therapeutic Medicine·Huixiang YaoJinshui Zhu
Jul 6, 2019·Journal of Diabetes and Metabolic Disorders·Ariel WilliamsK Sean Kimbro
Mar 12, 2019·BioMed Research International·Xiaodan BaiShixin Xu
Nov 20, 2020·Cells·Seley GharaneiHarpal S Randeva
Jun 12, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Xuemei LiShujun Zhao
Dec 17, 2019·Molecular Therapy. Nucleic Acids·Xiaomin LiuZhongliang Ma

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