miR‑17‑5p regulates the proliferation and apoptosis of human trabecular meshwork cells by targeting phosphatase and tensin homolog

Molecular Medicine Reports
Xiaoyuan WangFengmin Zhang

Abstract

Glaucoma is one of the leading causes of blindness. Previous studies have indicated that the oxidative stress‑induced apoptosis of trabecular meshwork cells (TMCs) may serve a key role in the pathogenesis of glaucoma, and that micro RNA(miR)‑17‑5p may be involved in this process. However, the specific mechanisms require further investigation. The aim of the present study was to investigate the effects of miR‑17‑5p on the proliferation and apoptosis of human TMCs (HTMCs) in response to oxidative stress. It was observed that exposure to H2O2 induced a significant decrease in the proliferation and a marked increase in the apoptosis of HTMCs. H2O2 exposure also suppressed the expression of miR‑17‑5p and promoted the expression of phosphatase and tensin homolog (PTEN). Furthermore, transient overexpression of miR‑17‑5p induced a significant increase in the proliferation and a significant decrease in the apoptosis of HTMCs by affecting the expression of PTEN, and the apoptosis‑related proteins B‑cell lymphoma‑associated X protein (Bax), B‑cell lymphoma‑extra large (Bcl‑xL) and B‑cell lymphoma‑2 (Bcl‑2). However, knockdown of miR‑17‑5p demonstrated the opposite results. The results of a dual luciferase reporter assay demonstrated that...Continue Reading

References

Oct 31, 2008·Investigative Ophthalmology & Visual Science·Alice L YuUlrich Welge-Lüssen
Sep 29, 2009·Mechanisms of Ageing and Development·Guorong LiPedro Gonzalez
Sep 3, 2011·Cancer Science·Akihiko TsuchidaMasahiko Kuroda
May 3, 2014·Journal of Glaucoma·Yuan Bo LiangRavi Thomas
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Methods Mentioned

BETA
PCR
flow cytometry
transfection

Software Mentioned

miRanda
SPSS
TargetScan

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