miR‑200b‑3p inhibits proliferation and induces apoptosis in colorectal cancer by targeting Wnt1

Molecular Medicine Reports
Lijuan ChenQingzhong Lai

Abstract

MicroRNA (miR)‑200b‑3p is downregulated in multiple human cancer types. Wnt signaling serves a role in human colorectal cancer (CRC). The present study aimed to examine the effect of miR‑200b‑3p on human CRC and its potential association with Wnt signaling. The Cell Counting Kit‑8 (CCK‑8) was employed to assess cell viability. A flow cytometric assay was conducted to examine cell proliferation and apoptosis. The regulation model of miR‑200b‑3p and Wnt1 was assessed by a luciferase reporter assay. A commercial kit was used to evaluate the activity of caspase‑3 following treatment of the cells by miR‑200b‑3p or Wnt1. The expression of target factors was determined by a quantitative real‑time polymerase chain reaction and western blot analysis. The expression of miR‑200b‑3p was decreased in human CRC tissues and in cell lines. The bioinformatics analysis and the luciferase reporter assay revealed that Wnt1 may be a direct target of miR‑200b‑3p. Moreover, the viability and proliferation of CRC cells was suppressed by miR‑200b‑3p. miR‑200b‑3p additionally induced apoptosis in CRC cells. Furthermore, the caspase‑3 activity was enhanced in the miR‑200b‑3p mimics group. The expression of antigen Ki‑67 (additionally termed KI‑67) and β‑...Continue Reading

References

Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Feb 7, 1998·Genes & Development·K M Cadigan, R Nusse
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Aug 17, 1999·Current Opinion in Genetics & Development·J Capdevila, J C lzpisúa Belmonte
Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 4, 2003·Current Opinion in Cell Biology·Kelly M Boatright, Guy S Salvesen
Apr 8, 2004·Neoplasia : an International Journal for Oncology Research·Biao HeDavid M Jablons
Sep 4, 2004·Genome Biology·Jing WangJulian A T Dow
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Jun 10, 2009·Journal of Medical Genetics·S GhavamiM Los
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward
Jul 8, 2011·Digestive Diseases·Alastair J M Watson, Paul D Collins
Oct 4, 2012·Indian Journal of Pathology & Microbiology·Xiaojun DaiLin Li
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Jan 7, 2014·PloS One·LaTanya V WilliamsOlga V Volpert
Feb 25, 2014·Biochimica Et Biophysica Acta·Aristeidis G VaiopoulosAthanasios G Papavassiliou
Oct 21, 2015·Oncotarget·Youn-Sang JungJae-Il Park
Aug 16, 2016·The American Journal of the Medical Sciences·Wentao SiQifeng Yang

❮ Previous
Next ❯

Citations

Jul 28, 2020·International Journal of Molecular Sciences·Ugne GyvyteJurgita Skieceviciene
Sep 15, 2020·Frontiers in Oncology·Xiaobo NieWei-Dong Chen
Jun 18, 2021·Frontiers in Pharmacology·Hongdan HeHengxiu Yan

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
Assay
PCR
X-ray

Software Mentioned

FACSDiva
GraphPad Prism
Quantity One

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.