miR‑205‑5p/PTK7 axis is involved in the proliferation, migration and invasion of colorectal cancer cells.

Molecular Medicine Reports
Shuo ChenXipeng Zhang

Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs, which are critical in a diverse range of biological processes, including development, differentiation, homeostasis, and in the formation of diseases by accelerating and/or inhibiting the translation of mRNAs. The present study aimed to examine the potential role of miRNA (miR)‑205‑5p in the developmental process of colorectal cancer (CRC) through protein‑tyrosine kinase 7 (PTK7). Initially, TargetScan was used to predict the miRNA target sites in the sequence of the PTK7 3'‑untranslated region. It was then found that the mRNA expression level of miR‑205‑5p was lower in CRC cells, determined using reverse transcription‑quantitative polymerase chain reaction analysis, and there was a negative correlation between miR‑205‑5p and PTK7 in CRC tissues. It was also found that miR‑205‑5p regulated the gene transcription of PTK7, determined using a luciferase reporter assay. The results of RT‑qPCR and western blot analyses in human colorectal cancer revealed that miR‑205‑5p suppressed the expression of PTK7. Finally, it was revealed that miR‑205‑5p restricted the proliferation ability of CRC cells through inhibiting PTK7, which was determined using colony forming and 3‑(4,5‑dimethylthiazol‑2‑...Continue Reading

References

Oct 13, 2001·Science·S SahaB Vogelstein
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 24, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carsten Müller-TidowHubert Serve
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hideki EndohTetsuya Mitsudomi
Jul 2, 2004·Nature·Xiaowei LuMarc Tessier-Lavigne
Sep 17, 2004·Nature·Victor Ambros
Dec 22, 2004·Genes, Chromosomes & Cancer·Kylie L GorringeUNKNOWN Melbourne Gastric Cancer Group, Peter Mac Micro Array Facility
Jul 13, 2005·Cell·Carlo M Croce, George A Calin
Oct 28, 2005·The New England Journal of Medicine·George Adrian CalinCarlo M Croce
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Sep 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Claudia RoldoCarlo M Croce
Apr 17, 2007·Diseases of the Colon and Rectum·Ghaith KhairJohn Greenman
May 13, 2008·Biochemical and Biophysical Research Communications·Won-Sik ShinSeung-Taek Lee
Nov 14, 2008·Development·Iryna Shnitsar, Annette Borchers
Nov 3, 2010·Clinics in Colon and Rectal Surgery·Fatima A Haggar, Robin P Boushey
Feb 5, 2011·Science·Sergej DjuranovicRachel Green
May 16, 2012·Journal of Surgical Oncology·Yi LinJia-Fu Ji
Dec 15, 2012·Journal of Ovarian Research·Mohd Saif ZamanSubhash C Chauhan
Jan 15, 2013·Cancer Research·Constance BaerChristoph Plass
May 15, 2013·Cancer Science·Won-Sik ShinJong Ho Park
Jun 21, 2013·Frontiers in Oncology·Gianpiero Di Leva, Carlo M Croce
Aug 29, 2013·BioMed Research International·Zhiyong ZhangTieqiao Wen
Jan 1, 2014·Genome Medicine·Carlos Stahlhut, Frank J Slack
Aug 17, 2014·Experimental Cell Research·Vinod GopalanAlfred K-Y Lam
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Aug 21, 2015·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·William D TravisUNKNOWN WHO Panel

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Methods Mentioned

BETA
transfections
PCR
Protein Assay
transfection
flow cytometry

Software Mentioned

FlowJo

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