miR‑215 promotes epithelial to mesenchymal transition and proliferation by regulating LEFTY2 in endometrial cancer

International Journal of Molecular Medicine
Xiaoxu GaoRuifang An

Abstract

Endometrial cancer (EC) is the most common gynecological tumor in developed countries with an increasing incidence. Left‑right determination factor 2 (LEFTY2), a suppressor of cell proliferation and tumor growth, is a negative regulator of EC progression. The roles of LEFTY2 are emerging; however, the regulatory mechanisms of its expression have not been well understood. MicroRNA (miR)‑215 as an oncogene serves an important role in tumorigenesis by regulating target genes. In the present study, it was demonstrated that overexpression of miR‑215 promoted epithelial to mesenchymal transition (EMT), colony formation and DNA synthesis in EC HEC‑1A cells and its expression was upregulated in EC tissues. Using online miR target prediction software, it was revealed that LEFTY2 is predicted as a target of miR‑215. Using western blot analysis and immunofluorescence assays, it was demonstrated that overexpression of miR‑215 markedly downregulated LEFTY2 protein expression levels in HEC‑1A cells and LEFTY2 protein expression was downregulated in EC tissues, which was inversely correlated with miR‑215 expression. Furthermore, the present study indicated that overexpression of LEFTY2 protein promoted mesenchymal to epithelial transition and...Continue Reading

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Citations

Feb 25, 2020·Journal of Cellular Physiology·Shiqi DengZhe Jin
Jul 17, 2020·Computational and Mathematical Methods in Medicine·Xinhua XuRongbiao Ying
Jul 31, 2020·Cancers·Monika SobočanJohannes Haybaeck
Apr 4, 2021·International Journal of Molecular Sciences·Roberto PiergentiliFederica Perelli

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Methods Mentioned

BETA
transfection
protein assay
PCR

Software Mentioned

ImageJ
miRanda

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