miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

Molecular Medicine Reports
Xiang SiXiang-Dong Guan

Abstract

Autophagy, part of the innate immune defense mechanisms, is activated during the initial phase of septic insult. Previous studies indicated that micro (mi)RNAs are additionally involved in the host response to sepsis; however, the association between miRNAs and autophagy during this process is not fully understood. To study the role of miRNA (miR)‑23a in autophagy initiated by sepsis, macrophages treated with lipopolysaccharides, in addition to blood samples from patients, were evaluated for miR‑23a expression levels. Cell viability, inflammatory mediators and autophagic markers were investigated following overexpression or inhibition of miR‑23a. The results suggested that miR‑23a was suppressed subsequent to septic insult, promoting autophagy and suppressing a hyper inflammatory response, leading to enhanced cell viability. A luciferase assay and western blot analysis confirmed ubiquitin‑like protein ATG12 to be the target of miR‑23a. The present study revealed that the downregulation of miR‑23a regulates an inflammatory response during septic insult via autophagy promotion.

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Citations

Sep 12, 2019·Frontiers in Physiology·Xin YinLiheng Guo
Feb 1, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Yunus Akkoc, Devrim Gozuacik

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Methods Mentioned

BETA
GTPase
transfection
ELISA

Software Mentioned

Linear Models of Microarray Analysis
heatmap
miRanda
R
Image
R studio

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