miR‑330‑5p inhibits H2O2‑induced adipogenic differentiation of MSCs by regulating RXRγ.

International Journal of Molecular Medicine
Weiping HuangHongqi Wang

Abstract

The elucidation of the underlying molecular mechanism of H2O2‑induced adipocyte differentiation in mesenchymal stem cells (MSCs) is important for the development of treatments for metabolic diseases. The aim of the present study was to identify microRNA (miR)‑330‑5p, which targets retinoid X receptor γ (RXRγ) and to determine the function of H2O2‑induced adipogenic differentiation of MSCs. During differentiation of MSCs into adipocytes induced by H2O2, miR‑330‑5p expression was decreased with a concomitant increase in RXRγ expression. A luciferase assay with RXRγ 3'‑untranslated region (UTR) reporter plasmid, including the miR‑330‑5p‑binding sequences, identified that the introduction of miR‑330‑5p decreases luciferase activity. However, it did not affect the activity of mutated RXRγ 3'‑UTR reporter. Enforced expression of miR‑330‑5p significantly inhibited adipocyte differentiation by decreasing RXRγ mRNA and protein levels. In contrast, inhibition of the endogenous miR‑330‑5p promoted the formation of lipid droplets by rescuing RXRγ expression. Furthermore, the effects of inhibition of RXRγ were similar to those of overexpression of miR‑330‑5p on H2O2‑induced adipogenic differentiation from MSCs. miR‑330‑5p inhibits H2O2‑indu...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 23, 2004·The EMBO Journal·Annemieke IJpenbergBéatrice Desvergne
Dec 16, 2004·The Journal of Clinical Investigation·Shigetada FurukawaIichiro Shimomura
Feb 3, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Atsushi NoharaHiroshi Mabuchi
Feb 25, 2009·The Journal of Biological Chemistry·Haemi LeeJae-Woo Kim
Jan 12, 2010·Biochemical and Biophysical Research Communications·Sang Yun KimJae Bum Kim
Dec 8, 2010·Molecular and Cellular Biology·Eun Kyung LeeMyriam Gorospe
Jul 5, 2011·Life Sciences·Yasunari KandaYasuhiro Watanabe
Oct 11, 2011·Cell Metabolism·Kathryn V TormosNavdeep S Chandel
May 1, 2012·Cell·Margaret S Ebert, Phillip A Sharp
Apr 1, 2014·Cell·Ronald M Evans, David J Mangelsdorf
Apr 23, 2014·Obesity·Per-Arne SvenssonMaria K Svensson
Oct 7, 2014·Journal of Cellular and Molecular Medicine·Cuiping MaoChangjun Lv
Feb 1, 2011·Journal of Developmental Origins of Health and Disease·B Blumberg
Jun 25, 2016·British Journal of Biomedical Science·Haniyeh FooladinezhadAbbas Doosti
Sep 14, 2017·Endocrine Reviews·Michael W SchwartzRudolph L Leibel

❮ Previous
Next ❯

Citations

Jun 25, 2021·Frontiers in Cell and Developmental Biology·Chao YangQiang Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
transfect
Oil Red O
dissection

Software Mentioned

GraphPad
ImageJ
SPSS
DAVID
miRWalk2
GraphPad Prism
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.