miR375-3p Distinguishes Low-Grade Neuroendocrine From Non-neuroendocrine Lung Tumors in FFPE Samples

Frontiers in Molecular Biosciences
Simone DetassisMichela Alessandra Denti

Abstract

Lung cancer is still one of the leading cause of death worldwide. The clinical variability of lung cancer is high and drives treatment decision. In this context, correct discrimination of pulmonary neuroendocrine tumors is still of critical relevance. The spectrum of neuroendocrine tumors is various, and each type has molecular and phenotypical differences. In order to advance in the discrimination of neuroendocrine from non-neuroendocrine lung tumors, we tested a series of 95 surgically resected and formalin-fixed paraffin embedded lung cancer tissues, and we analyzed the expression of miR205-5p and miR375-3p via TaqMan RT-qPCR. Via a robust mathematical approach, we excluded technical outliers increasing the data reproducibility. We found that miR375-3p levels are higher in low-grade neuroendocrine lung tumor samples compared to non-neuroendocrine lung tumors. However, miR375-3p is not able to distinguish among different types of neuroendocrine lung tumors. In this work, we provide a new molecular marker for distinguishing non-neuroendocrine from low-grade neuroendocrine lung tumors samples establishing an easy miRNA score to be used in clinical settings, enabling the pathologist to classify more accurately lung tumors biopsi...Continue Reading

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Citations

Jan 29, 2021·International Journal of Molecular Sciences·Francesca PrecazziniPaola Campomenosi
Jul 3, 2021·Non-coding RNA·Alexandra KorotaevaAlexander Karpukhin
Jan 25, 2022·Experimental and Therapeutic Medicine·Cornel SavuNicolae Bacalbasa

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Methods Mentioned

BETA
biopsies
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PCR

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