miR‑486‑5p is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by suppressing SMAD2

Molecular Medicine Reports
Junkai ShiChunhui Li

Abstract

Osteoarthritis (OA) is the most common form of arthritis and is caused by the breakdown of joint cartilage. The present study aimed to investigate an effective method for the treatment of OA. It was demonstrated that, compared with other patients, patients with OA exhibited lower mRNA expression levels of SMAD family member 2 (SMAD2). MicroRNA (miR)‑486‑5p was predicted to bind with SMAD2, which was verified by dual‑luciferase reporter assay. Compared withcontrol patients who had no known history of OA or rheumatoid arthritis, patients with OA exhibited higher miR‑486‑5p expression level. Treatment with miR‑486‑5p mimics inhibited proliferation and migration of CHON‑001 human chondrocytes, and also inhibited the expression levels of type II collagen and aggrecan. However, treatment with a miR‑486‑5p inhibitor promoted proliferation and migration, and the expression of type II collagen and aggrecan. Short interfering RNA‑directed silencing of SMAD2 reversed the upregulated proliferation and migration and the expression level of type II collagen and aggrecan induced by the miR‑486‑5p inhibitor. In conclusion, the results of the present study indicated that miR‑486‑5p was upregulated in OA and may inhibit chondrocyte proliferation...Continue Reading

References

Jul 1, 1996·Nature Genetics·G J RigginsB Vogelstein
Mar 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·R ItayemF P Reinholt
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 17, 2004·Nature·Victor Ambros
Mar 30, 2007·Osteoarthritis and Cartilage·E N Blaney DavidsonW B van den Berg
Mar 13, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kenneth W FinnsonAnie Philip
Oct 13, 2010·Biochemical and Biophysical Research Communications·Kumi SumiyoshiMasaharu Takigawa
Nov 3, 2010·The Journal of Biological Chemistry·Fethi LouafiTilman Sanchez-Elsner
Aug 8, 2014·The Journal of Bone and Joint Surgery. American Volume·Constance R ChuSteven A Olson
Sep 4, 2014·Current Rheumatology Reports·Johannah Sanchez-AdamsFarshid Guilak
Dec 8, 2014·Best Practice & Research. Clinical Rheumatology·Lyn MarchAnthony D Woolf
Mar 10, 2015·Lancet·S Glyn-JonesA J Carr

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.